Exposure of HeLa cells to 06-alkylguanines increases sensitivity to the cytotoxic effects of alkylating agents

M. Eileen Dolan, Christopher D. Corsico, Anthony E. Pegg

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Exposure of HeLa S3 cells to 0.4mM 06-methylguanine or 06-n-butylguanine for 24 h led to a substantial decrease in the activity of 06-alkylguanine-DNA alkyltransferase. Such pretreatment caused a marked increase in the sensitivity of the cells to the cytotoxic effects of the cross-linking alkylating agent 1-(2-chloroethyl)-1-nitroso-3-cyclohexylurea and a smaller increase in the sensitivity to N-methyl-N′-nitro-N-nitrosoguanidine. These results indicate that the repair of DNA by the alkyltransferase plays an important role in the protection of cells from the cytotoxic effects of certain alkylating agents particularly those such as 1-(2-chloroethyl)-1-nitroso-3-cyclohexylurea which ultimately lead to the formation of lethal interstrand cross-links.

Original languageEnglish (US)
Pages (from-to)178-185
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Oct 15 1985

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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