Exposure of herpes simplex virus-infected cells to excimer laser photocoagulation

C. M. Mcrili, Wallace Greene, K. P. Kolanda, G. Ü, D. Rosenwasser

Research output: Contribution to journalArticle

Abstract

Recent concerns over the safety of operator exposure to excimer laser plume prompted this study We sought to determine if live Herpes simplex type I (HSV-1 i could survive exposure to a routine excimer laser pulse sequence MRC-.5 cell monolayers were infected with wild-type HSV-1 in culture tubes to a concenlration of HI7 TCID (tissue culture infective doses). Cells were scraped to dislodge them and cenlrifuged to a pellel. The pellets were resuspended and subjected to a pulse sequence equal to a -VOO diopter correction (150 pulses with a 6.5mrn optical /one. Summit Technologies SVS Apex Excimer Laser System]. The laser plume from 10 samples and a negative control was collected via vacuum into a small vial containing tissue culture medium Fresh cell monolayers were immediately inoculated with the collecling medium. No virus grew from any of the 10 samples inoculated wiih HSV-1 or from the negative conlrol. A pooled collection medium was sent to a reference lab for PCR detection of viral DNA. Viral DNA was found by PCR in Ihe pooled sample but not from the negative control. In conclusion, viable HSV-1 could not be recovered from excimer laser plume under these conditions, but viral DNA fragments were detected by PCR. Studies are underway to further characterize the PCR products.

Original languageEnglish (US)
Pages (from-to)S533
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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