TY - JOUR
T1 - Exposure of Lung Epithelial Cells to Photochemically Aged Secondary Organic Aerosol Shows Increased Toxic Effects
AU - Chowdhury, Pratiti Home
AU - He, Quanfu
AU - Lasitza Male, Tatyana
AU - Brune, William H.
AU - Rudich, Yinon
AU - Pardo, Michal
N1 - Funding Information:
This study was partially funded by grants from the Germany-Israel Science Foundation (GIF Grant I-38-307.1-2015), the Israel Ministry of Science, Technology and Space (Maimonide program), and Astrachan Olga Klein.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/7/10
Y1 - 2018/7/10
N2 - Adverse health effects due to exposure to particulate matter (PM) are among the most important global environmental health risks. However, the effects of exposure to secondary organic aerosols (SOA), a major component of the global aerosol, are largely unknown. Here we exposed lung epithelial cells (A549) to fresh and aged SOA particles and investigated the effect of SOA atmospheric aging on cell viability and gene expression. Naphthalene- and α-pinene-derived SOA were formed in an oxidation flow reactor that simulates atmospheric SOA formation and aging dominated by OH radical oxidation under NOx-free conditions. The SOA mass and chemical composition were characterized on-line using a scanning mobility particle sizer and aerosol mass spectrometer. Fresh and aged SOA were directed to an air-liquid interface cell exposure system. Aged naphthalene- and α-pinene-derived SOA were somewhat more toxic than fresh SOA. Aged naphthalene SOA contained peroxide levels that were higher than those of fresh SOA. The level of induction of Nrf2 signaling increased following exposure to aged naphthalene SOA. Given the global prevalence of SOA and its observed toxicity, this study calls for more studies aimed at understanding the underlying mechanics.
AB - Adverse health effects due to exposure to particulate matter (PM) are among the most important global environmental health risks. However, the effects of exposure to secondary organic aerosols (SOA), a major component of the global aerosol, are largely unknown. Here we exposed lung epithelial cells (A549) to fresh and aged SOA particles and investigated the effect of SOA atmospheric aging on cell viability and gene expression. Naphthalene- and α-pinene-derived SOA were formed in an oxidation flow reactor that simulates atmospheric SOA formation and aging dominated by OH radical oxidation under NOx-free conditions. The SOA mass and chemical composition were characterized on-line using a scanning mobility particle sizer and aerosol mass spectrometer. Fresh and aged SOA were directed to an air-liquid interface cell exposure system. Aged naphthalene- and α-pinene-derived SOA were somewhat more toxic than fresh SOA. Aged naphthalene SOA contained peroxide levels that were higher than those of fresh SOA. The level of induction of Nrf2 signaling increased following exposure to aged naphthalene SOA. Given the global prevalence of SOA and its observed toxicity, this study calls for more studies aimed at understanding the underlying mechanics.
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U2 - 10.1021/acs.estlett.8b00256
DO - 10.1021/acs.estlett.8b00256
M3 - Article
AN - SCOPUS:85048726267
SN - 2328-8930
VL - 5
SP - 424
EP - 430
JO - Environmental Science and Technology Letters
JF - Environmental Science and Technology Letters
IS - 7
ER -
