Expressed sequence tag analysis of the soybean rust pathogen Phakopsora pachyrhizi

Martha Lucia Posada-Buitrago, Reid David Frederick

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Soybean rust is caused by the obligate fungal pathogen Phakopsora pachyrhizi Sydow. A unidirectional cDNA library was constructed using mRNA isolated from germinating P. pachyrhizi urediniospores to identify genes expressed at this physiological stage. Single pass sequence analysis of 908 clones revealed 488 unique expressed sequence tags (ESTs, unigenes) of which 107 appeared as multiple copies. BLASTX analysis identified 189 unigenes with significant similarities (Evalue < 10-5) to sequences deposited in the NCBI non-redundant protein database. A search against the NCBI dbEST using the BLASTN algorithm revealed 32 ESTs with high or moderate similarities to plant and fungal sequences. Using the Expressed Gene Anatomy Classification, 31.7% of these ESTs were involved in primary metabolism, 14.3% in gene/protein expression, 7.4% in cell structure and growth, 6.9% in cell division, 4.8% in cell signaling/cell communication, and 4.8% in cell/organism defense. Approximately 29.6% of the identities were to hypothetical proteins and proteins with unknown function.

Original languageEnglish (US)
Pages (from-to)949-962
Number of pages14
JournalFungal Genetics and Biology
Volume42
Issue number12
DOIs
StatePublished - Dec 1 2005

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Expressed Sequence Tags
Soybeans
Sequence Analysis
Protein Databases
Proteins
Gene Library
Cell Communication
Cell Division
Genes
Anatomy
Clone Cells
Gene Expression
Messenger RNA
Phakopsora pachyrhizi
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

Posada-Buitrago, Martha Lucia ; Frederick, Reid David. / Expressed sequence tag analysis of the soybean rust pathogen Phakopsora pachyrhizi. In: Fungal Genetics and Biology. 2005 ; Vol. 42, No. 12. pp. 949-962.
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Expressed sequence tag analysis of the soybean rust pathogen Phakopsora pachyrhizi. / Posada-Buitrago, Martha Lucia; Frederick, Reid David.

In: Fungal Genetics and Biology, Vol. 42, No. 12, 01.12.2005, p. 949-962.

Research output: Contribution to journalArticle

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