Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa

Mary Anne Nelson, Seogchan Kang, Edward L. Braun, Matthew E. Crawford, Patricia L. Dolan, Pascale M. Leonard, Jason Mitchell, Anne Marie Armijo, Laura Bean, Eldon Blueyes, Thomas Cushing, Allison Errett, Mark Fleharty, Marianita Gorman, Kimberly Judson, Robert Miller, Jennifer Ortega, Iglika Pavlova, John Perea, Shanda TodiscoRobert Trujillo, Joseph Valentine, Audrey Wells, Margaret Werner-Washburne, Sheldwin Yazzie, Donald O. Natvig

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1865 partial complementary DNA (cDNA) sequences for 1409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34% of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56% of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3% were involved in metabolism, 32.9% in protein synthesis and 8.4% in RNA synthesis. Fewer were involved in defense (6%), cell signalling (3.4%), cell structure (3.4%) and cell division (2.6%).

Original languageEnglish (US)
Pages (from-to)348-363
Number of pages16
JournalFungal Genetics and Biology
Volume21
Issue number3
DOIs
StatePublished - Jan 1 1997

Fingerprint

Neurospora crassa
Expressed Sequence Tags
Complementary DNA
Clone Cells
Genes
Neurospora
Life Cycle Stages
Cell Division
Fungi
Genome
Databases
RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Genetics

Cite this

Nelson, M. A., Kang, S., Braun, E. L., Crawford, M. E., Dolan, P. L., Leonard, P. M., ... Natvig, D. O. (1997). Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa. Fungal Genetics and Biology, 21(3), 348-363. https://doi.org/10.1006/fgbi.1997.0986
Nelson, Mary Anne ; Kang, Seogchan ; Braun, Edward L. ; Crawford, Matthew E. ; Dolan, Patricia L. ; Leonard, Pascale M. ; Mitchell, Jason ; Armijo, Anne Marie ; Bean, Laura ; Blueyes, Eldon ; Cushing, Thomas ; Errett, Allison ; Fleharty, Mark ; Gorman, Marianita ; Judson, Kimberly ; Miller, Robert ; Ortega, Jennifer ; Pavlova, Iglika ; Perea, John ; Todisco, Shanda ; Trujillo, Robert ; Valentine, Joseph ; Wells, Audrey ; Werner-Washburne, Margaret ; Yazzie, Sheldwin ; Natvig, Donald O. / Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa. In: Fungal Genetics and Biology. 1997 ; Vol. 21, No. 3. pp. 348-363.
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abstract = "In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1865 partial complementary DNA (cDNA) sequences for 1409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34{\%} of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56{\%} of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3{\%} were involved in metabolism, 32.9{\%} in protein synthesis and 8.4{\%} in RNA synthesis. Fewer were involved in defense (6{\%}), cell signalling (3.4{\%}), cell structure (3.4{\%}) and cell division (2.6{\%}).",
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Nelson, MA, Kang, S, Braun, EL, Crawford, ME, Dolan, PL, Leonard, PM, Mitchell, J, Armijo, AM, Bean, L, Blueyes, E, Cushing, T, Errett, A, Fleharty, M, Gorman, M, Judson, K, Miller, R, Ortega, J, Pavlova, I, Perea, J, Todisco, S, Trujillo, R, Valentine, J, Wells, A, Werner-Washburne, M, Yazzie, S & Natvig, DO 1997, 'Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa', Fungal Genetics and Biology, vol. 21, no. 3, pp. 348-363. https://doi.org/10.1006/fgbi.1997.0986

Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa. / Nelson, Mary Anne; Kang, Seogchan; Braun, Edward L.; Crawford, Matthew E.; Dolan, Patricia L.; Leonard, Pascale M.; Mitchell, Jason; Armijo, Anne Marie; Bean, Laura; Blueyes, Eldon; Cushing, Thomas; Errett, Allison; Fleharty, Mark; Gorman, Marianita; Judson, Kimberly; Miller, Robert; Ortega, Jennifer; Pavlova, Iglika; Perea, John; Todisco, Shanda; Trujillo, Robert; Valentine, Joseph; Wells, Audrey; Werner-Washburne, Margaret; Yazzie, Sheldwin; Natvig, Donald O.

In: Fungal Genetics and Biology, Vol. 21, No. 3, 01.01.1997, p. 348-363.

Research output: Contribution to journalArticle

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T1 - Expressed sequences from conidial, mycelial, and sexual stages of Neurospora crassa

AU - Nelson, Mary Anne

AU - Kang, Seogchan

AU - Braun, Edward L.

AU - Crawford, Matthew E.

AU - Dolan, Patricia L.

AU - Leonard, Pascale M.

AU - Mitchell, Jason

AU - Armijo, Anne Marie

AU - Bean, Laura

AU - Blueyes, Eldon

AU - Cushing, Thomas

AU - Errett, Allison

AU - Fleharty, Mark

AU - Gorman, Marianita

AU - Judson, Kimberly

AU - Miller, Robert

AU - Ortega, Jennifer

AU - Pavlova, Iglika

AU - Perea, John

AU - Todisco, Shanda

AU - Trujillo, Robert

AU - Valentine, Joseph

AU - Wells, Audrey

AU - Werner-Washburne, Margaret

AU - Yazzie, Sheldwin

AU - Natvig, Donald O.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - In the Neurospora Genome Project at the University of New Mexico, expressed sequence tags (ESTs) corresponding to three stages of the life cycle of the filamentous fungus Neurospora crassa are being analyzed. The results of a pilot project to identify expressed genes and determine their patterns of expression are presented. 1865 partial complementary DNA (cDNA) sequences for 1409 clones were determined using single-pass sequencing. Contig analysis allowed the identification of 838 unique ESTs and 156 ESTs present in multiple cDNA clones. For about 34% of the sequences, highly or moderately significant matches to sequences (of known and unknown function) in the NCBI database were detected. Approximately 56% of the ESTs showed no similarity to previously identified genes. Among genes with assigned function, about 43.3% were involved in metabolism, 32.9% in protein synthesis and 8.4% in RNA synthesis. Fewer were involved in defense (6%), cell signalling (3.4%), cell structure (3.4%) and cell division (2.6%).

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