Expression and regulation of cytochrome P450 17α-hydroxylase messenger ribonucleic acid levels and androstenedione production in hen granulosa cells

Z. Li, Alan Leslie Johnson

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31 Scopus citations

Abstract

Studies were conducted to evaluate the expression and regulation of cytochrome P450 17α-hydroxylase (P450 17α-OH) mRNA levels and androstenedione production in 6-8-mm-diameter follicle granulosa cells from the domestic hen. Although P450 17α-OH mRNA was detected within granulosa cells from follicles at all stages of development (3-12-mm and preovulatory follicles), 8-12-fold higher levels were found in small, less developed follicles (3-12 mm in diameter) compared to the three largest follicles within the preovulatory hierarchy (F3 to F1 follicles). By comparison, P450 17α-OH mRNA levels were 25-89-fold higher in theca tissue compared to the granulosa layer at comparable stages of development. Despite detection of P450 17α-OH mRNA in granulosa cells from 6-8-mm follicles, androstenedione production was low to nondetectable when cells were cultured in the presence of exogenous progesterone (10 ng/ml). Treatment with FSH increased levels of P450 17α-OH mRNA (by 6-fold after 16 h of treatment) and induced androstenedione production in cultured granulosa cells; these actions were mimicked by the cAMP analog, 8-bromo-cAMP. By contrast, addition of the growth factors transforming growth factor α (TGFα), epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF) completely suppressed the stimulatory effects of FSH on both mRNA levels and androstenedione production, while insulin-like growth factor I (IGF-I) inhibited only androstenedione production. We conclude that in the granulosa layer of developing (≤ 12 mm) hen follicles the expression of P450 17α-OH mRNA does not directly reflect P450 17α-OH enzyme activity. Moreover, P450 17α-OH mRNA levels dramatically decrease in granulosa cells from preovulatory (compared to developing) follicles, and in the theca layer from the F1 preovulatory follicle (compared to remaining follicles). It is proposed that FSH initiates P450 17α-OH enzyme activity (and perhaps to a lesser extent regulates mRNA levels) at the time a follicle is recruited into the preovulatory hierarchy, and that this action is mediated, at least in part, by the adenylyl cyclase/cAMP second messenger pathway. On the other hand, growth factors (including TGFα, EGF, bFGF, and possibly IGF-I) may act to prevent premature expression of P450 17α-OH activity in the granulosa layer of follicles that have yet to enter the rapid growth phase of follicle development.

Original languageEnglish (US)
Pages (from-to)1293-1302
Number of pages10
JournalBiology of reproduction
Volume49
Issue number6
DOIs
StatePublished - Nov 30 1993

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

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