Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

Stanley Person, Susan C. Warner, David J. Bzik, Chitrita Debroy, Barbara A. Fox

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

Original languageEnglish (US)
Pages (from-to)279-287
Number of pages9
JournalGene
Volume35
Issue number3
DOIs
StatePublished - Jan 1 1985

Fingerprint

Human Herpesvirus 2
Glycoproteins
Bacteria
Peptides
Virology
Inclusion Bodies
Human Herpesvirus 1
Plasmids
Clone Cells
Escherichia coli
Amino Acids
Antibodies
Genes

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Person, S., Warner, S. C., Bzik, D. J., Debroy, C., & Fox, B. A. (1985). Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2. Gene, 35(3), 279-287. https://doi.org/10.1016/0378-1119(85)90006-X
Person, Stanley ; Warner, Susan C. ; Bzik, David J. ; Debroy, Chitrita ; Fox, Barbara A. / Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2. In: Gene. 1985 ; Vol. 35, No. 3. pp. 279-287.
@article{3a3d5ebc8dad41c3bc32e1ca9737bd07,
title = "Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2",
abstract = "A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].",
author = "Stanley Person and Warner, {Susan C.} and Bzik, {David J.} and Chitrita Debroy and Fox, {Barbara A.}",
year = "1985",
month = "1",
day = "1",
doi = "10.1016/0378-1119(85)90006-X",
language = "English (US)",
volume = "35",
pages = "279--287",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "3",

}

Person, S, Warner, SC, Bzik, DJ, Debroy, C & Fox, BA 1985, 'Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2', Gene, vol. 35, no. 3, pp. 279-287. https://doi.org/10.1016/0378-1119(85)90006-X

Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2. / Person, Stanley; Warner, Susan C.; Bzik, David J.; Debroy, Chitrita; Fox, Barbara A.

In: Gene, Vol. 35, No. 3, 01.01.1985, p. 279-287.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

AU - Person, Stanley

AU - Warner, Susan C.

AU - Bzik, David J.

AU - Debroy, Chitrita

AU - Fox, Barbara A.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

AB - A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

UR - http://www.scopus.com/inward/record.url?scp=0022263096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022263096&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(85)90006-X

DO - 10.1016/0378-1119(85)90006-X

M3 - Article

VL - 35

SP - 279

EP - 287

JO - Gene

JF - Gene

SN - 0378-1119

IS - 3

ER -