Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2

Stanley Person, Susan C. Warner, David J. Bzik, Chitrita Debroy, Barbara A. Fox

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZα. expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].

Original languageEnglish (US)
Pages (from-to)279-287
Number of pages9
JournalGene
Volume35
Issue number3
DOIs
StatePublished - 1985

All Science Journal Classification (ASJC) codes

  • Genetics

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