Expression of a 32 kDa protein in rat mammary tumors induced by anti-benzo[c]phenanthrene-3,4-diol-1,2-epoxide

Ze'ev Ronai, Alla Polotskaya, Scott Gradia, Shantu Amin, Stephen S. Hecht

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Racemic anti-benzo[c]phenanthrene-3,4-diol-1,2-epoxide (BcPDE) is a powerful rat mammary carcinogen and a metabolite of benzo[c]phenanthrene, a polynuclear aromatic hydrocarbon found in the environment. In elucidating potential molecular mechanisms that may play a role in the development of BcPDE-induced rat mammary tumors, we have identified a 32 kDa protein in 16 of 26 tumors analyzed but in only 1 of the 15 normal mammary tissues that were examined. The 32 kDa protein was identified with antibodies to Ets, which also recognized the 55 kDa Ets-1 protein that was expressed at similar levels in normal mammary tissues. The expression of the 32 kDa protein was also observed in mammary tumor-derived cell lines of both rat and human origin and in human melanoma, but not in normal human keratinocytes or rat fibroblast cell lines. Further characterization via 20 gels revealed that the protein exhibits a PI of 5.5. Southwestern analysis using Ets-1 target sequence revealed binding of the 55 kDa Ets-1 but not of the newly identified 32 kDa protein. Overall, the preferential expression of the 32 kDa protein in mammary tumor tissues may serve as a biomarker to follow the development of this tumor type.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalInternational Journal of Cancer
Volume67
Issue number1
DOIs
StatePublished - Jul 29 1996

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Epoxy Compounds
Breast Neoplasms
Proteins
Breast
Polycyclic Aromatic Hydrocarbons
benzo(c)phenanthrene
Tumor Cell Line
Keratinocytes
Carcinogens
Melanoma
Neoplasms
Fibroblasts
Biomarkers
Gels
Cell Line
Antibodies

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

Ronai, Ze'ev ; Polotskaya, Alla ; Gradia, Scott ; Amin, Shantu ; Hecht, Stephen S. / Expression of a 32 kDa protein in rat mammary tumors induced by anti-benzo[c]phenanthrene-3,4-diol-1,2-epoxide. In: International Journal of Cancer. 1996 ; Vol. 67, No. 1. pp. 124-128.
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Expression of a 32 kDa protein in rat mammary tumors induced by anti-benzo[c]phenanthrene-3,4-diol-1,2-epoxide. / Ronai, Ze'ev; Polotskaya, Alla; Gradia, Scott; Amin, Shantu; Hecht, Stephen S.

In: International Journal of Cancer, Vol. 67, No. 1, 29.07.1996, p. 124-128.

Research output: Contribution to journalArticle

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