Expression of bcl-2 and nr-13 in hen ovarian follicles during development

Alan Leslie Johnson, J. T. Bridgham, J. P. Witty, J. L. Tilly

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Follicle atresia is initiated within the granulosa cell layer of ovarian follicles and is mediated via the process of apoptosis. In the hen, at least two populations of granulosa cells can be distinguished during follicle development on the basis of their inherent susceptibility or resistance to apoptosis, in vitro. Given the previously established correlation between expression of bcl-x(long) and hen granulosa cell resistance to apoptosis, the present studies were conducted to characterize expression of bcl-2 and an avian bcl-2 homologue, nr-13, in follicles at various stages of development. Levels of nr-13 mRNA were significantly higher only in granulosa cells from the largest (F1) preovulatory follicle compared to 3- to 5-mm prehierarchal follicles. By comparison, bcl-2 mRNA levels were 5- to 9-fold higher in granulosa cells from the three largest preovulatory follicles compared to those from follicles 9 to 12 mm in diameter and prehierarchal follicles. The increase in neither nr-13 nor bcl-2 was correlated with the stage of follicle development associated with the acquisition of resistance to apoptosis in granulosa cells (e.g., at the 9- to 12-mm stage). Results from the present studies do not support a close correlation between constitutive expression of nr-13 or bcl-2 mRNA and the transition from a state of apoptosis susceptibility to apoptosis resistance in hen granulosa cells. Thus, it is proposed that nr-13 and bcl-2 play more of a supportive role in regulating additional aspects of ovarian cell function such as cell proliferation and/or differentiation.

Original languageEnglish (US)
Pages (from-to)1096-1103
Number of pages8
JournalBiology of Reproduction
Volume57
Issue number5
DOIs
StatePublished - Nov 1 1997

Fingerprint

Ovarian Follicle
Granulosa Cells
Apoptosis
Messenger RNA
Cell Differentiation
Cell Proliferation
Population

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Johnson, Alan Leslie ; Bridgham, J. T. ; Witty, J. P. ; Tilly, J. L. / Expression of bcl-2 and nr-13 in hen ovarian follicles during development. In: Biology of Reproduction. 1997 ; Vol. 57, No. 5. pp. 1096-1103.
@article{f19b3feed7d54e37b0d04c1d47df5181,
title = "Expression of bcl-2 and nr-13 in hen ovarian follicles during development",
abstract = "Follicle atresia is initiated within the granulosa cell layer of ovarian follicles and is mediated via the process of apoptosis. In the hen, at least two populations of granulosa cells can be distinguished during follicle development on the basis of their inherent susceptibility or resistance to apoptosis, in vitro. Given the previously established correlation between expression of bcl-x(long) and hen granulosa cell resistance to apoptosis, the present studies were conducted to characterize expression of bcl-2 and an avian bcl-2 homologue, nr-13, in follicles at various stages of development. Levels of nr-13 mRNA were significantly higher only in granulosa cells from the largest (F1) preovulatory follicle compared to 3- to 5-mm prehierarchal follicles. By comparison, bcl-2 mRNA levels were 5- to 9-fold higher in granulosa cells from the three largest preovulatory follicles compared to those from follicles 9 to 12 mm in diameter and prehierarchal follicles. The increase in neither nr-13 nor bcl-2 was correlated with the stage of follicle development associated with the acquisition of resistance to apoptosis in granulosa cells (e.g., at the 9- to 12-mm stage). Results from the present studies do not support a close correlation between constitutive expression of nr-13 or bcl-2 mRNA and the transition from a state of apoptosis susceptibility to apoptosis resistance in hen granulosa cells. Thus, it is proposed that nr-13 and bcl-2 play more of a supportive role in regulating additional aspects of ovarian cell function such as cell proliferation and/or differentiation.",
author = "Johnson, {Alan Leslie} and Bridgham, {J. T.} and Witty, {J. P.} and Tilly, {J. L.}",
year = "1997",
month = "11",
day = "1",
doi = "10.1095/biolreprod57.5.1096",
language = "English (US)",
volume = "57",
pages = "1096--1103",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "5",

}

Expression of bcl-2 and nr-13 in hen ovarian follicles during development. / Johnson, Alan Leslie; Bridgham, J. T.; Witty, J. P.; Tilly, J. L.

In: Biology of Reproduction, Vol. 57, No. 5, 01.11.1997, p. 1096-1103.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of bcl-2 and nr-13 in hen ovarian follicles during development

AU - Johnson, Alan Leslie

AU - Bridgham, J. T.

AU - Witty, J. P.

AU - Tilly, J. L.

PY - 1997/11/1

Y1 - 1997/11/1

N2 - Follicle atresia is initiated within the granulosa cell layer of ovarian follicles and is mediated via the process of apoptosis. In the hen, at least two populations of granulosa cells can be distinguished during follicle development on the basis of their inherent susceptibility or resistance to apoptosis, in vitro. Given the previously established correlation between expression of bcl-x(long) and hen granulosa cell resistance to apoptosis, the present studies were conducted to characterize expression of bcl-2 and an avian bcl-2 homologue, nr-13, in follicles at various stages of development. Levels of nr-13 mRNA were significantly higher only in granulosa cells from the largest (F1) preovulatory follicle compared to 3- to 5-mm prehierarchal follicles. By comparison, bcl-2 mRNA levels were 5- to 9-fold higher in granulosa cells from the three largest preovulatory follicles compared to those from follicles 9 to 12 mm in diameter and prehierarchal follicles. The increase in neither nr-13 nor bcl-2 was correlated with the stage of follicle development associated with the acquisition of resistance to apoptosis in granulosa cells (e.g., at the 9- to 12-mm stage). Results from the present studies do not support a close correlation between constitutive expression of nr-13 or bcl-2 mRNA and the transition from a state of apoptosis susceptibility to apoptosis resistance in hen granulosa cells. Thus, it is proposed that nr-13 and bcl-2 play more of a supportive role in regulating additional aspects of ovarian cell function such as cell proliferation and/or differentiation.

AB - Follicle atresia is initiated within the granulosa cell layer of ovarian follicles and is mediated via the process of apoptosis. In the hen, at least two populations of granulosa cells can be distinguished during follicle development on the basis of their inherent susceptibility or resistance to apoptosis, in vitro. Given the previously established correlation between expression of bcl-x(long) and hen granulosa cell resistance to apoptosis, the present studies were conducted to characterize expression of bcl-2 and an avian bcl-2 homologue, nr-13, in follicles at various stages of development. Levels of nr-13 mRNA were significantly higher only in granulosa cells from the largest (F1) preovulatory follicle compared to 3- to 5-mm prehierarchal follicles. By comparison, bcl-2 mRNA levels were 5- to 9-fold higher in granulosa cells from the three largest preovulatory follicles compared to those from follicles 9 to 12 mm in diameter and prehierarchal follicles. The increase in neither nr-13 nor bcl-2 was correlated with the stage of follicle development associated with the acquisition of resistance to apoptosis in granulosa cells (e.g., at the 9- to 12-mm stage). Results from the present studies do not support a close correlation between constitutive expression of nr-13 or bcl-2 mRNA and the transition from a state of apoptosis susceptibility to apoptosis resistance in hen granulosa cells. Thus, it is proposed that nr-13 and bcl-2 play more of a supportive role in regulating additional aspects of ovarian cell function such as cell proliferation and/or differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0030807949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030807949&partnerID=8YFLogxK

U2 - 10.1095/biolreprod57.5.1096

DO - 10.1095/biolreprod57.5.1096

M3 - Article

VL - 57

SP - 1096

EP - 1103

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 5

ER -