Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus

Shu Shan Zhao, Xiao Mei Li, Xiang Pei Li, Zhi Min Zhai, Zhu Chen, Yan Ma, Hong Zhang, Song Guo Zheng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To detect the new and old surface markers of regulatory T cells (Treg cells) in the CD4+ T cells of the patients with systemic lupus erythematosus (SLE) in order to reveal the role of Treg cells in the pathogenesis of SLE. Methods: Peripheral blood samples were collected from 29 newly diagnosed and treatment-naïve SLE patients, 3 males and 26 females, aged (34 ± 13), and 24 sex and aged-matched healthy controls. Three-color flow cytometry was used to detect the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25high T cells, and CD4+ CD25+ FOXP3+ T cells. The serum anti-nuclear antibody (ANA), anti-ds-DNA antibody, anti-smooth muscle antibody, anti-nucleosome antibody, anti-C1q, C3, and C4 were detected. Blood and urine routine examinations were conducted. Results: The proportion of blood CD4+ CD25+ CD127low/ - T cells of the SLE patients was not significantly different from that of the controls (P > 0.05), however, the proportions of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cells of the SLE patients were 2.1 ± 1.2 and 0.8 ± 0.4 respectively, both significantly lower than those of the controls (4.0 ± 1.4 and 1.8 ± 0.8 respectively, both P < 0.01). The ratios of the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25highT cells, and CD4+ CD25+ FOXP3+ T cells to the CD4+ CD25+ T cells of the SLE patients were 0.5 ± 0.1, 0.1 ± 0, and 0.3 ± 0.1 respectively, all significantly lower than those of the controls (0.6 ± 0.1, 0.2 ± 0.1 and 0.5 ± 0. respectively, all P < 0.01). The level of CD4+ CD25+ CD127low/ - T cell was positively correlated with the levels of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cell (both P < 0.01). The levels of these 3 kinds of cells and their ratios to CD4+ CD25+ T cells had no correlation with age, sex, course, IgG, IgA, IgM, urine protein, TIPU, anti-dsDNA, anti-C1q, anti-nuclear body antibody (all P > 0.05), however, were significantly associated negatively with SLE disease activity index, P < 0.05). Only the CD4+ CD25+ CD127low/ - T cells/CD4+ CD25+ T cells was negatively correlated with C4 (P < 0.01). Conclusion: The relative ratio of Treg cells to the activated CD4+ T cells may play an important role in the pathogenesis of SLE.

Original languageEnglish (US)
Pages (from-to)453-456
Number of pages4
JournalNational Medical Journal of China
Volume88
Issue number7
StatePublished - Feb 19 2008

Fingerprint

Systemic Lupus Erythematosus
T-Lymphocytes
Regulatory T-Lymphocytes
Anti-Idiotypic Antibodies
Nucleosomes
Antinuclear Antibodies
Smooth Muscle
Flow Cytometry
Color
Urine
DNA
Serum

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Zhao, S. S., Li, X. M., Li, X. P., Zhai, Z. M., Chen, Z., Ma, Y., ... Zheng, S. G. (2008). Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus. National Medical Journal of China, 88(7), 453-456.
Zhao, Shu Shan ; Li, Xiao Mei ; Li, Xiang Pei ; Zhai, Zhi Min ; Chen, Zhu ; Ma, Yan ; Zhang, Hong ; Zheng, Song Guo. / Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus. In: National Medical Journal of China. 2008 ; Vol. 88, No. 7. pp. 453-456.
@article{3a056e7b2baf4d2b890889a86d959bd7,
title = "Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus",
abstract = "Objective: To detect the new and old surface markers of regulatory T cells (Treg cells) in the CD4+ T cells of the patients with systemic lupus erythematosus (SLE) in order to reveal the role of Treg cells in the pathogenesis of SLE. Methods: Peripheral blood samples were collected from 29 newly diagnosed and treatment-na{\"i}ve SLE patients, 3 males and 26 females, aged (34 ± 13), and 24 sex and aged-matched healthy controls. Three-color flow cytometry was used to detect the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25high T cells, and CD4+ CD25+ FOXP3+ T cells. The serum anti-nuclear antibody (ANA), anti-ds-DNA antibody, anti-smooth muscle antibody, anti-nucleosome antibody, anti-C1q, C3, and C4 were detected. Blood and urine routine examinations were conducted. Results: The proportion of blood CD4+ CD25+ CD127low/ - T cells of the SLE patients was not significantly different from that of the controls (P > 0.05), however, the proportions of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cells of the SLE patients were 2.1 ± 1.2 and 0.8 ± 0.4 respectively, both significantly lower than those of the controls (4.0 ± 1.4 and 1.8 ± 0.8 respectively, both P < 0.01). The ratios of the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25highT cells, and CD4+ CD25+ FOXP3+ T cells to the CD4+ CD25+ T cells of the SLE patients were 0.5 ± 0.1, 0.1 ± 0, and 0.3 ± 0.1 respectively, all significantly lower than those of the controls (0.6 ± 0.1, 0.2 ± 0.1 and 0.5 ± 0. respectively, all P < 0.01). The level of CD4+ CD25+ CD127low/ - T cell was positively correlated with the levels of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cell (both P < 0.01). The levels of these 3 kinds of cells and their ratios to CD4+ CD25+ T cells had no correlation with age, sex, course, IgG, IgA, IgM, urine protein, TIPU, anti-dsDNA, anti-C1q, anti-nuclear body antibody (all P > 0.05), however, were significantly associated negatively with SLE disease activity index, P < 0.05). Only the CD4+ CD25+ CD127low/ - T cells/CD4+ CD25+ T cells was negatively correlated with C4 (P < 0.01). Conclusion: The relative ratio of Treg cells to the activated CD4+ T cells may play an important role in the pathogenesis of SLE.",
author = "Zhao, {Shu Shan} and Li, {Xiao Mei} and Li, {Xiang Pei} and Zhai, {Zhi Min} and Zhu Chen and Yan Ma and Hong Zhang and Zheng, {Song Guo}",
year = "2008",
month = "2",
day = "19",
language = "English (US)",
volume = "88",
pages = "453--456",
journal = "Zhonghua yi xue za zhi",
issn = "0376-2491",
publisher = "Zhonghua Yixuehui Zazhishe",
number = "7",

}

Zhao, SS, Li, XM, Li, XP, Zhai, ZM, Chen, Z, Ma, Y, Zhang, H & Zheng, SG 2008, 'Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus', National Medical Journal of China, vol. 88, no. 7, pp. 453-456.

Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus. / Zhao, Shu Shan; Li, Xiao Mei; Li, Xiang Pei; Zhai, Zhi Min; Chen, Zhu; Ma, Yan; Zhang, Hong; Zheng, Song Guo.

In: National Medical Journal of China, Vol. 88, No. 7, 19.02.2008, p. 453-456.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus

AU - Zhao, Shu Shan

AU - Li, Xiao Mei

AU - Li, Xiang Pei

AU - Zhai, Zhi Min

AU - Chen, Zhu

AU - Ma, Yan

AU - Zhang, Hong

AU - Zheng, Song Guo

PY - 2008/2/19

Y1 - 2008/2/19

N2 - Objective: To detect the new and old surface markers of regulatory T cells (Treg cells) in the CD4+ T cells of the patients with systemic lupus erythematosus (SLE) in order to reveal the role of Treg cells in the pathogenesis of SLE. Methods: Peripheral blood samples were collected from 29 newly diagnosed and treatment-naïve SLE patients, 3 males and 26 females, aged (34 ± 13), and 24 sex and aged-matched healthy controls. Three-color flow cytometry was used to detect the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25high T cells, and CD4+ CD25+ FOXP3+ T cells. The serum anti-nuclear antibody (ANA), anti-ds-DNA antibody, anti-smooth muscle antibody, anti-nucleosome antibody, anti-C1q, C3, and C4 were detected. Blood and urine routine examinations were conducted. Results: The proportion of blood CD4+ CD25+ CD127low/ - T cells of the SLE patients was not significantly different from that of the controls (P > 0.05), however, the proportions of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cells of the SLE patients were 2.1 ± 1.2 and 0.8 ± 0.4 respectively, both significantly lower than those of the controls (4.0 ± 1.4 and 1.8 ± 0.8 respectively, both P < 0.01). The ratios of the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25highT cells, and CD4+ CD25+ FOXP3+ T cells to the CD4+ CD25+ T cells of the SLE patients were 0.5 ± 0.1, 0.1 ± 0, and 0.3 ± 0.1 respectively, all significantly lower than those of the controls (0.6 ± 0.1, 0.2 ± 0.1 and 0.5 ± 0. respectively, all P < 0.01). The level of CD4+ CD25+ CD127low/ - T cell was positively correlated with the levels of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cell (both P < 0.01). The levels of these 3 kinds of cells and their ratios to CD4+ CD25+ T cells had no correlation with age, sex, course, IgG, IgA, IgM, urine protein, TIPU, anti-dsDNA, anti-C1q, anti-nuclear body antibody (all P > 0.05), however, were significantly associated negatively with SLE disease activity index, P < 0.05). Only the CD4+ CD25+ CD127low/ - T cells/CD4+ CD25+ T cells was negatively correlated with C4 (P < 0.01). Conclusion: The relative ratio of Treg cells to the activated CD4+ T cells may play an important role in the pathogenesis of SLE.

AB - Objective: To detect the new and old surface markers of regulatory T cells (Treg cells) in the CD4+ T cells of the patients with systemic lupus erythematosus (SLE) in order to reveal the role of Treg cells in the pathogenesis of SLE. Methods: Peripheral blood samples were collected from 29 newly diagnosed and treatment-naïve SLE patients, 3 males and 26 females, aged (34 ± 13), and 24 sex and aged-matched healthy controls. Three-color flow cytometry was used to detect the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25high T cells, and CD4+ CD25+ FOXP3+ T cells. The serum anti-nuclear antibody (ANA), anti-ds-DNA antibody, anti-smooth muscle antibody, anti-nucleosome antibody, anti-C1q, C3, and C4 were detected. Blood and urine routine examinations were conducted. Results: The proportion of blood CD4+ CD25+ CD127low/ - T cells of the SLE patients was not significantly different from that of the controls (P > 0.05), however, the proportions of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cells of the SLE patients were 2.1 ± 1.2 and 0.8 ± 0.4 respectively, both significantly lower than those of the controls (4.0 ± 1.4 and 1.8 ± 0.8 respectively, both P < 0.01). The ratios of the CD4+ CD25+ CD127low/ - T cells, CD4+ CD25highT cells, and CD4+ CD25+ FOXP3+ T cells to the CD4+ CD25+ T cells of the SLE patients were 0.5 ± 0.1, 0.1 ± 0, and 0.3 ± 0.1 respectively, all significantly lower than those of the controls (0.6 ± 0.1, 0.2 ± 0.1 and 0.5 ± 0. respectively, all P < 0.01). The level of CD4+ CD25+ CD127low/ - T cell was positively correlated with the levels of CD4+ CD25+ FOXP3+ T cells and CD4+ CD25highT cell (both P < 0.01). The levels of these 3 kinds of cells and their ratios to CD4+ CD25+ T cells had no correlation with age, sex, course, IgG, IgA, IgM, urine protein, TIPU, anti-dsDNA, anti-C1q, anti-nuclear body antibody (all P > 0.05), however, were significantly associated negatively with SLE disease activity index, P < 0.05). Only the CD4+ CD25+ CD127low/ - T cells/CD4+ CD25+ T cells was negatively correlated with C4 (P < 0.01). Conclusion: The relative ratio of Treg cells to the activated CD4+ T cells may play an important role in the pathogenesis of SLE.

UR - http://www.scopus.com/inward/record.url?scp=43749101793&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43749101793&partnerID=8YFLogxK

M3 - Article

C2 - 18642784

AN - SCOPUS:43749101793

VL - 88

SP - 453

EP - 456

JO - Zhonghua yi xue za zhi

JF - Zhonghua yi xue za zhi

SN - 0376-2491

IS - 7

ER -

Zhao SS, Li XM, Li XP, Zhai ZM, Chen Z, Ma Y et al. Expression of CD4+ CD25+ CD127low/- T cells in patients with systemic lupus erythematosus. National Medical Journal of China. 2008 Feb 19;88(7):453-456.