Expression of DNA binding proteins in carrot somatic embryos that specifically interact with a cis regulatory element of the French bean phaseolin gene

Mark Guiltinan, John C. Thomas, Craig L. Nessler, Terry L. Thomas

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Nuclear proteins from bean (Phaseolus vulgarus) embryos bind specifically to a 55 bp DNA sequence located upstream of the seed storage protein gene phaseolin. This sequence is capable of elevating gene expression in transgenic tobacco plants by as much as 150-fold when fused to a chimeric β-glucuronidase reporter gene. Results presented in this paper demonstrate that nuclear extracts from carrot embryos bind to a phaseolin DNA sequence that includes a phaseolin activator sequence. This specific DNA binding activity is modulated during somatic embryogenesis. Two separable protein species react specifically with the labeled phaseolin DNA fragment (58.0 and 51.7 dDa). These results suggest that the cis- and trans-acting elements controlling gene expression have been highly conserved during evolution.

Original languageEnglish (US)
Pages (from-to)605-610
Number of pages6
JournalPlant Molecular Biology
Volume13
Issue number5
DOIs
StatePublished - Nov 1 1989

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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