Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris

Hiroko Shike, C. Shimizu, K. S. Klimpel, J. C. Burns

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The ability to introduce and express foreign genes can greatly facilitate in vitro studies of the molecular biology of an organism. We report here the first use of modified retroviral vectors to achieve foreign gene expression in primary cultured cells from the Oka organ and ovary of the blue shrimp Penaeus stylirostris (Litopenaeus) stylirostris (Holthuis 1980). Of the heterologous promoters tested, the Moloney murine leukemia virus and Rous sarcoma virus promoters mediated the highest levels of reporter gene expression. Firefly luciferase and Escherichia coli β-galactosidase were successfully used as reporter genes in cultured cells from both Oka organ and ovary. These in vitro systems can be exploited for the study of shrimp cellular biology and regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)605-611
Number of pages7
JournalMarine Biology
Volume137
Issue number4
DOIs
StatePublished - Jan 1 2000

Fingerprint

Litopenaeus stylirostris
reporter genes
cultured cells
gene expression
shrimp
promoter regions
Murine leukemia virus
retroviral vectors
Rous sarcoma virus
galactosidases
gene
virus
gene expression regulation
luciferase
cell biology
in vitro studies
molecular biology
genes
Escherichia coli
organisms

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Cite this

Shike, Hiroko ; Shimizu, C. ; Klimpel, K. S. ; Burns, J. C. / Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris. In: Marine Biology. 2000 ; Vol. 137, No. 4. pp. 605-611.
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Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris. / Shike, Hiroko; Shimizu, C.; Klimpel, K. S.; Burns, J. C.

In: Marine Biology, Vol. 137, No. 4, 01.01.2000, p. 605-611.

Research output: Contribution to journalArticle

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