Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris

Hiroko Shike, C. Shimizu, K. S. Klimpel, J. C. Burns

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The ability to introduce and express foreign genes can greatly facilitate in vitro studies of the molecular biology of an organism. We report here the first use of modified retroviral vectors to achieve foreign gene expression in primary cultured cells from the Oka organ and ovary of the blue shrimp Penaeus stylirostris (Litopenaeus) stylirostris (Holthuis 1980). Of the heterologous promoters tested, the Moloney murine leukemia virus and Rous sarcoma virus promoters mediated the highest levels of reporter gene expression. Firefly luciferase and Escherichia coli β-galactosidase were successfully used as reporter genes in cultured cells from both Oka organ and ovary. These in vitro systems can be exploited for the study of shrimp cellular biology and regulation of gene expression.

Original languageEnglish (US)
Pages (from-to)605-611
Number of pages7
JournalMarine Biology
Volume137
Issue number4
DOIs
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Fingerprint Dive into the research topics of 'Expression of foreign genes in primary cultured cells of the blue shrimp Penaeus stylirostris'. Together they form a unique fingerprint.

Cite this