Expression of FOXP3 in CD4 + CD39 + T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid

Dong Mei Li, Xiang Pei Li, Xiao Mei Li, Guo Sheng Wang, Yan Ma, Shu Shan Zhao, Song Guo Zheng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To investigate the level of FOXP3 expressed in CD4 + CD39 + T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4 + CD25 + CD39 + , CD4 + CD25 + FOXP3 + and CD4 + CD39 + FOXP3 + T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4 + CD25 + CD39 + T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4 + CD25 + , CD4 + CD25 high and CD4 + CD39 + T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4 + CD25 high and CD4 + CD39 + T cells were higher than that expressed in CD4 + CD25 + T cells (P < 0.01), while it had no significant difference between CD4 + CD25 high T cells and CD4 + CD39 + T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39 + Treg cells may play an important role in the pathogenesis of SLE.

Original languageEnglish (US)
Pages (from-to)1636-1638
Number of pages3
JournalNational Medical Journal of China
Volume89
Issue number23
DOIs
StatePublished - Jun 16 2009

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Systemic Lupus Erythematosus
Glucocorticoids
Observation
T-Lymphocytes
Therapeutics
Regulatory T-Lymphocytes
Flow Cytometry
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Li, Dong Mei ; Li, Xiang Pei ; Li, Xiao Mei ; Wang, Guo Sheng ; Ma, Yan ; Zhao, Shu Shan ; Zheng, Song Guo. / Expression of FOXP3 in CD4 + CD39 + T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid In: National Medical Journal of China. 2009 ; Vol. 89, No. 23. pp. 1636-1638.
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title = "Expression of FOXP3 in CD4 + CD39 + T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid",
abstract = "Objective: To investigate the level of FOXP3 expressed in CD4 + CD39 + T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4 + CD25 + CD39 + , CD4 + CD25 + FOXP3 + and CD4 + CD39 + FOXP3 + T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4 + CD25 + CD39 + T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5){\%}, (1.9 ± 0.8){\%} and (2.3 ± 1.0){\%} respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4 + CD25 + , CD4 + CD25 high and CD4 + CD39 + T cells were (45 ± 12){\%}, (65 ± 14){\%} and (70 ± 14){\%} respectively. Levels of FOXP3 expressed in CD4 + CD25 high and CD4 + CD39 + T cells were higher than that expressed in CD4 + CD25 + T cells (P < 0.01), while it had no significant difference between CD4 + CD25 high T cells and CD4 + CD39 + T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39 + Treg cells may play an important role in the pathogenesis of SLE.",
author = "Li, {Dong Mei} and Li, {Xiang Pei} and Li, {Xiao Mei} and Wang, {Guo Sheng} and Yan Ma and Zhao, {Shu Shan} and Zheng, {Song Guo}",
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Expression of FOXP3 in CD4 + CD39 + T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid . / Li, Dong Mei; Li, Xiang Pei; Li, Xiao Mei; Wang, Guo Sheng; Ma, Yan; Zhao, Shu Shan; Zheng, Song Guo.

In: National Medical Journal of China, Vol. 89, No. 23, 16.06.2009, p. 1636-1638.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of FOXP3 in CD4 + CD39 + T cells of patients with systemic lupus erythematosus and dynamic observation of treatment with glucocorticoid

AU - Li, Dong Mei

AU - Li, Xiang Pei

AU - Li, Xiao Mei

AU - Wang, Guo Sheng

AU - Ma, Yan

AU - Zhao, Shu Shan

AU - Zheng, Song Guo

PY - 2009/6/16

Y1 - 2009/6/16

N2 - Objective: To investigate the level of FOXP3 expressed in CD4 + CD39 + T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4 + CD25 + CD39 + , CD4 + CD25 + FOXP3 + and CD4 + CD39 + FOXP3 + T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4 + CD25 + CD39 + T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4 + CD25 + , CD4 + CD25 high and CD4 + CD39 + T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4 + CD25 high and CD4 + CD39 + T cells were higher than that expressed in CD4 + CD25 + T cells (P < 0.01), while it had no significant difference between CD4 + CD25 high T cells and CD4 + CD39 + T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39 + Treg cells may play an important role in the pathogenesis of SLE.

AB - Objective: To investigate the level of FOXP3 expressed in CD4 + CD39 + T cells in peripheral blood of patients with systemic lupus erythematosus (SLE) and observe the regulation of glucocorticoid on it. Methods: Frequencies of CD4 + CD25 + CD39 + , CD4 + CD25 + FOXP3 + and CD4 + CD39 + FOXP3 + T cells and levels of FOXP3 protein were analyzed by flow cytometry of 47 SLE patients (including 29 untreated/active SLE) and 22 healthy controls. Meanwhile, correlations among three groups and influences of glucocorticoid were analyzed. Results: Percents of CD4 + CD25 + CD39 + T cells expressed in active SLE, inactive SLE and healthy controls were (1.3 ± 0.5)%, (1.9 ± 0.8)% and (2.3 ± 1.0)% respectively, the level decreased in active SLE compared with inactive SLE and healthy controls P < 0.05 in each group, but it had no significant difference between the latter two groups (P > 0.05). In active SLE, levels of FOXP3 protein expressed in CD4 + CD25 + , CD4 + CD25 high and CD4 + CD39 + T cells were (45 ± 12)%, (65 ± 14)% and (70 ± 14)% respectively. Levels of FOXP3 expressed in CD4 + CD25 high and CD4 + CD39 + T cells were higher than that expressed in CD4 + CD25 + T cells (P < 0.01), while it had no significant difference between CD4 + CD25 high T cells and CD4 + CD39 + T cells (P > 0.05). Conclusions: These results demonstrate that CD39 may be a better surface marker of regulatory T cells, and that deficiency of CD39 + Treg cells may play an important role in the pathogenesis of SLE.

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U2 - 10.3760/cma.j.issn.0376-2491.2009.23.015

DO - 10.3760/cma.j.issn.0376-2491.2009.23.015

M3 - Article

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EP - 1638

JO - Zhonghua yi xue za zhi

JF - Zhonghua yi xue za zhi

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