Expression of GPR177 (Wntless/Evi/Sprinter), a highly conserved Wnt-transport protein, in rat tissues, zebrafish embryos, and cultured human cells

Jay Jin, Megan Morse, Colleen Frey, Jessica A. Petko, Robert Levenson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

GPR177 is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. Little is currently known, however, regarding expression of GPR177, especially in vertebrate species. We have developed an antiserum against GPR177, and used it to examine expression of GPR177 in human tissue culture cells, adult mouse, and rat tissues, as well as developing zebrafish embryos. In rodents, GPR177 is expressed in virtually all tissue types and brain regions examined. In zebrafish, GPR177 polypeptides are expressed throughout embryogenesis, and are detectable as early as 1 hr post-fertilization. In situ hybridization analysis reveals that gpr177 mRNA expression is prominent in embryonic zebrafish brain and ear. Structural studies suggest that GPR177 is modified by N-linked sugars, and that the protein contains an even number of transmembrane segments. The relatively ubiquitous expression of GPR177 suggests that this protein may serve to regulate Wnt secretion in a variety of embryonic and adult tissue types.

Original languageEnglish (US)
Pages (from-to)2426-2434
Number of pages9
JournalDevelopmental Dynamics
Volume239
Issue number9
DOIs
StatePublished - Sep 1 2010

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Wnt Proteins
Zebrafish
Cultured Cells
Carrier Proteins
Embryonic Structures
Proteins
Brain
Fertilization
Embryonic Development
In Situ Hybridization
Ear
Vertebrates
Immune Sera
Rodentia
Cell Culture Techniques
Messenger RNA
Peptides

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

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Expression of GPR177 (Wntless/Evi/Sprinter), a highly conserved Wnt-transport protein, in rat tissues, zebrafish embryos, and cultured human cells. / Jin, Jay; Morse, Megan; Frey, Colleen; Petko, Jessica A.; Levenson, Robert.

In: Developmental Dynamics, Vol. 239, No. 9, 01.09.2010, p. 2426-2434.

Research output: Contribution to journalArticle

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