Expression of mouse-GLUT3 and human-GLUT3 glucose transporter proteins in brain

Frances Maher, Susan Vannucci, Jun Takeda, Ian Simpson

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Abstract

Polyclonal anti-peptide antisera were raised to the C-terminal sequence of mouse- and human-GLUT3 glucose transporter isoforms. GLUT3 protein and mRNA expression were investigated by Western blot and Northern blot assays, in a range of tissues and cell lines. Mouse-GLUT3 protein was detected only in rat brain, where it was present in most regions except adenohypophysis and pineal gland. Mouse-GLUT3 was also detected in primary cultured rat cerebellar neurons and the neuronal cell lines PC12 and NG108-15, but not in cultured astroglia. Human-GLUT3 protein was detected in CHO cells transfected with the human-GLUT3 cDNA and in human brain, but not in human placenta or fat. The expression of GLUT3 in rat brain and neurons indicates it is a major neuronal glucose transporter.

Original languageEnglish (US)
Pages (from-to)703-711
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number2
DOIs
StatePublished - Jan 31 1992

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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