Expression of phycobiliprotein genes in Escherichia coli

D. A. Bryant, J. M. Dubbs, P. I. Fields, R. D. Porter, R. de Lorimier

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The cpcA and cpcB genes, which encode the α- and β-subunit apoproteins of Synechococcus 7002 phycocyanin (PC), respectively, are efficiently expressed in Escherichia coli. Studies performed with a series of deletions produced from the parental plasmid pAQPR1 suggest that transcription of these genes in E. coli is initiated from a promoter located not more than 384 bp upstream from the translation start of cpcB. An examination of the nucleotide sequence of this region reveals a consensus E. coli promoter centered approx. 374 bp upstream from the cpcB translation start. The cyanelle-encoded allophycocyanin α- and β-subunit apoprotein genes (apcA and apcB) of Cyanophora paradoxa are also expressed in E. coli.

Original languageEnglish (US)
Pages (from-to)343-349
Number of pages7
JournalFEMS Microbiology Letters
Volume29
Issue number3
DOIs
StatePublished - Sep 1985

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

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