Expression of rat apolipoprotein A-IV and A-I genes: mRNA induction during development and in response to glucocorticoids and insulin

N. A. Elshourbagy, M. S. Boguski, W. S.L. Liao, Leonard "Jim" Jefferson, J. I. Gordon, J. M. Taylor

Research output: Contribution to journalArticle

170 Citations (Scopus)

Abstract

Rat apolipoproteins (apo-) A-IV and A-I share many structural similarities, the most notable of which is a domain of repeated docosapeptides with amphipathic helical potential. Although the genes for apo-A-IV and apo-A-I probably diverged from a common ancestor, these proteins seem to have developed different functions in their evolution. In the present study, clone cDNAs were used to characterize the expression of apo-A-IV and apo-A-I mRNAs in a wide variety of adult rat tissues, as well as in small intestine and liver obtained from fetal, suckling, and weanling animals; comparisons were made to the the expression of apo-E mRNA. The apo-A-IV and apo-A-I mRNAs were most abundant in adult small intestine and liver, with trace amounts detected in other tissues. Substantial amounts of these mRNAs were detected in the yolk sac, suggesting that this fetal tissue plays an important role in lipid metabolism during gestation. Noncoordinate accumulation of apo-A-IV and apo-A-I mRNAs was observed within and between the liver and small intestine during neonatal development. The apo-A-IV mRNA levels in the developing small intestine and liver appeared to correlate with their triglyceride secretion rates, suggesting that this protein plays an important role in the metabolism of triglyceride-rich lipoproteins. When dexamethasone (0.1 μM), insulin (0.01 μM), or insulin and dexamethasone together were incubated with primary cultures of nonproliferating adult rat hepatocytes, apo-A-IV mRNA levels were 4-,7- and 11-fold higher, respectively, than in non-hormone-treated control hepatocytes. Hormone administration resulted in a 2-fold greater amount of apo-A-I mRNA in each case, with no significant change in the level of apo-E mRNA. The overall results suggest that these structurally related apolipoproteins are regulated in substantially different ways.

Original languageEnglish (US)
Pages (from-to)8242-8246
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number23
DOIs
StatePublished - Dec 1 1985

Fingerprint

Glucocorticoids
Insulin
Apolipoprotein A-I
Messenger RNA
Genes
Small Intestine
Liver
Apolipoproteins E
Dexamethasone
Suckling Animals
Hepatocytes
Triglycerides
apolipoprotein A-IV
Yolk Sac
Apolipoproteins
Lipid Metabolism
Lipoproteins
Proteins
Fetus
Complementary DNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{77a468375dab42c9a06ecab6e9113909,
title = "Expression of rat apolipoprotein A-IV and A-I genes: mRNA induction during development and in response to glucocorticoids and insulin",
abstract = "Rat apolipoproteins (apo-) A-IV and A-I share many structural similarities, the most notable of which is a domain of repeated docosapeptides with amphipathic helical potential. Although the genes for apo-A-IV and apo-A-I probably diverged from a common ancestor, these proteins seem to have developed different functions in their evolution. In the present study, clone cDNAs were used to characterize the expression of apo-A-IV and apo-A-I mRNAs in a wide variety of adult rat tissues, as well as in small intestine and liver obtained from fetal, suckling, and weanling animals; comparisons were made to the the expression of apo-E mRNA. The apo-A-IV and apo-A-I mRNAs were most abundant in adult small intestine and liver, with trace amounts detected in other tissues. Substantial amounts of these mRNAs were detected in the yolk sac, suggesting that this fetal tissue plays an important role in lipid metabolism during gestation. Noncoordinate accumulation of apo-A-IV and apo-A-I mRNAs was observed within and between the liver and small intestine during neonatal development. The apo-A-IV mRNA levels in the developing small intestine and liver appeared to correlate with their triglyceride secretion rates, suggesting that this protein plays an important role in the metabolism of triglyceride-rich lipoproteins. When dexamethasone (0.1 μM), insulin (0.01 μM), or insulin and dexamethasone together were incubated with primary cultures of nonproliferating adult rat hepatocytes, apo-A-IV mRNA levels were 4-,7- and 11-fold higher, respectively, than in non-hormone-treated control hepatocytes. Hormone administration resulted in a 2-fold greater amount of apo-A-I mRNA in each case, with no significant change in the level of apo-E mRNA. The overall results suggest that these structurally related apolipoproteins are regulated in substantially different ways.",
author = "Elshourbagy, {N. A.} and Boguski, {M. S.} and Liao, {W. S.L.} and Jefferson, {Leonard {"}Jim{"}} and Gordon, {J. I.} and Taylor, {J. M.}",
year = "1985",
month = "12",
day = "1",
doi = "10.1073/pnas.82.23.8242",
language = "English (US)",
volume = "82",
pages = "8242--8246",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "23",

}

Expression of rat apolipoprotein A-IV and A-I genes : mRNA induction during development and in response to glucocorticoids and insulin. / Elshourbagy, N. A.; Boguski, M. S.; Liao, W. S.L.; Jefferson, Leonard "Jim"; Gordon, J. I.; Taylor, J. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 23, 01.12.1985, p. 8242-8246.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of rat apolipoprotein A-IV and A-I genes

T2 - mRNA induction during development and in response to glucocorticoids and insulin

AU - Elshourbagy, N. A.

AU - Boguski, M. S.

AU - Liao, W. S.L.

AU - Jefferson, Leonard "Jim"

AU - Gordon, J. I.

AU - Taylor, J. M.

PY - 1985/12/1

Y1 - 1985/12/1

N2 - Rat apolipoproteins (apo-) A-IV and A-I share many structural similarities, the most notable of which is a domain of repeated docosapeptides with amphipathic helical potential. Although the genes for apo-A-IV and apo-A-I probably diverged from a common ancestor, these proteins seem to have developed different functions in their evolution. In the present study, clone cDNAs were used to characterize the expression of apo-A-IV and apo-A-I mRNAs in a wide variety of adult rat tissues, as well as in small intestine and liver obtained from fetal, suckling, and weanling animals; comparisons were made to the the expression of apo-E mRNA. The apo-A-IV and apo-A-I mRNAs were most abundant in adult small intestine and liver, with trace amounts detected in other tissues. Substantial amounts of these mRNAs were detected in the yolk sac, suggesting that this fetal tissue plays an important role in lipid metabolism during gestation. Noncoordinate accumulation of apo-A-IV and apo-A-I mRNAs was observed within and between the liver and small intestine during neonatal development. The apo-A-IV mRNA levels in the developing small intestine and liver appeared to correlate with their triglyceride secretion rates, suggesting that this protein plays an important role in the metabolism of triglyceride-rich lipoproteins. When dexamethasone (0.1 μM), insulin (0.01 μM), or insulin and dexamethasone together were incubated with primary cultures of nonproliferating adult rat hepatocytes, apo-A-IV mRNA levels were 4-,7- and 11-fold higher, respectively, than in non-hormone-treated control hepatocytes. Hormone administration resulted in a 2-fold greater amount of apo-A-I mRNA in each case, with no significant change in the level of apo-E mRNA. The overall results suggest that these structurally related apolipoproteins are regulated in substantially different ways.

AB - Rat apolipoproteins (apo-) A-IV and A-I share many structural similarities, the most notable of which is a domain of repeated docosapeptides with amphipathic helical potential. Although the genes for apo-A-IV and apo-A-I probably diverged from a common ancestor, these proteins seem to have developed different functions in their evolution. In the present study, clone cDNAs were used to characterize the expression of apo-A-IV and apo-A-I mRNAs in a wide variety of adult rat tissues, as well as in small intestine and liver obtained from fetal, suckling, and weanling animals; comparisons were made to the the expression of apo-E mRNA. The apo-A-IV and apo-A-I mRNAs were most abundant in adult small intestine and liver, with trace amounts detected in other tissues. Substantial amounts of these mRNAs were detected in the yolk sac, suggesting that this fetal tissue plays an important role in lipid metabolism during gestation. Noncoordinate accumulation of apo-A-IV and apo-A-I mRNAs was observed within and between the liver and small intestine during neonatal development. The apo-A-IV mRNA levels in the developing small intestine and liver appeared to correlate with their triglyceride secretion rates, suggesting that this protein plays an important role in the metabolism of triglyceride-rich lipoproteins. When dexamethasone (0.1 μM), insulin (0.01 μM), or insulin and dexamethasone together were incubated with primary cultures of nonproliferating adult rat hepatocytes, apo-A-IV mRNA levels were 4-,7- and 11-fold higher, respectively, than in non-hormone-treated control hepatocytes. Hormone administration resulted in a 2-fold greater amount of apo-A-I mRNA in each case, with no significant change in the level of apo-E mRNA. The overall results suggest that these structurally related apolipoproteins are regulated in substantially different ways.

UR - http://www.scopus.com/inward/record.url?scp=0008138455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0008138455&partnerID=8YFLogxK

U2 - 10.1073/pnas.82.23.8242

DO - 10.1073/pnas.82.23.8242

M3 - Article

C2 - 3934672

AN - SCOPUS:0008138455

VL - 82

SP - 8242

EP - 8246

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -