Expression of the p16(INK4a) gene product in premalignant and malignant epithelial lesions of the gallbladder

Bridget C. Lynch, Sarah L. Lathrop, Dongmei Ye, Thomas Y. Ma, Lisa A. Cerilli

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The sequence of molecular changes leading to neoplastic transformation in the gallbladder remains elusive. The aim of this study is to characterize the spectrum of nuclear p16 protein product immunohistochemical expression in tissue microarray cores taken from resected gallbladders, comprising histologically normal gallbladder epithelia (n = 29), dyplastic epithelia (n = 19), reactive atypia (n = 7), and gallbladder adenocarcinoma (n = 23). Nuclear staining for p16 was evaluated for intensity (range, 0-3) and distribution (range, 0-3), and a summary staining score (range, 0-6) was obtained. Pairwise comparisons were significant for increased p16 protein expression in dysplastic epithelium (6/19, 31.6%) and invasive carcinoma (9/23, 39.1%) when compared to normal epithelium (0/29, 0%) (P = .003 and P = .0006, respectively). A quantitative increase of nuclear expression of p16 in reactive atypia (1/7, 14%) when compared to normal was also statistically significant (P = .049). Our data demonstrate that nuclear p16 expression is absent in normal gallbladder epithelium and is a frequent event in high-grade dysplasia of the gallbladder and gallbladder adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)161-164
Number of pages4
JournalAnnals of Diagnostic Pathology
Volume12
Issue number3
DOIs
StatePublished - Jun 1 2008

Fingerprint

p16 Genes
Gallbladder
Epithelium
Adenocarcinoma
Staining and Labeling
Nuclear Proteins
Carcinoma

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Lynch, Bridget C. ; Lathrop, Sarah L. ; Ye, Dongmei ; Ma, Thomas Y. ; Cerilli, Lisa A. / Expression of the p16(INK4a) gene product in premalignant and malignant epithelial lesions of the gallbladder. In: Annals of Diagnostic Pathology. 2008 ; Vol. 12, No. 3. pp. 161-164.
@article{65c78b22649e4f4b826a3009de19bffd,
title = "Expression of the p16(INK4a) gene product in premalignant and malignant epithelial lesions of the gallbladder",
abstract = "The sequence of molecular changes leading to neoplastic transformation in the gallbladder remains elusive. The aim of this study is to characterize the spectrum of nuclear p16 protein product immunohistochemical expression in tissue microarray cores taken from resected gallbladders, comprising histologically normal gallbladder epithelia (n = 29), dyplastic epithelia (n = 19), reactive atypia (n = 7), and gallbladder adenocarcinoma (n = 23). Nuclear staining for p16 was evaluated for intensity (range, 0-3) and distribution (range, 0-3), and a summary staining score (range, 0-6) was obtained. Pairwise comparisons were significant for increased p16 protein expression in dysplastic epithelium (6/19, 31.6{\%}) and invasive carcinoma (9/23, 39.1{\%}) when compared to normal epithelium (0/29, 0{\%}) (P = .003 and P = .0006, respectively). A quantitative increase of nuclear expression of p16 in reactive atypia (1/7, 14{\%}) when compared to normal was also statistically significant (P = .049). Our data demonstrate that nuclear p16 expression is absent in normal gallbladder epithelium and is a frequent event in high-grade dysplasia of the gallbladder and gallbladder adenocarcinoma.",
author = "Lynch, {Bridget C.} and Lathrop, {Sarah L.} and Dongmei Ye and Ma, {Thomas Y.} and Cerilli, {Lisa A.}",
year = "2008",
month = "6",
day = "1",
doi = "10.1016/j.anndiagpath.2007.06.003",
language = "English (US)",
volume = "12",
pages = "161--164",
journal = "Annals of Diagnostic Pathology",
issn = "1092-9134",
publisher = "W.B. Saunders Ltd",
number = "3",

}

Expression of the p16(INK4a) gene product in premalignant and malignant epithelial lesions of the gallbladder. / Lynch, Bridget C.; Lathrop, Sarah L.; Ye, Dongmei; Ma, Thomas Y.; Cerilli, Lisa A.

In: Annals of Diagnostic Pathology, Vol. 12, No. 3, 01.06.2008, p. 161-164.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Expression of the p16(INK4a) gene product in premalignant and malignant epithelial lesions of the gallbladder

AU - Lynch, Bridget C.

AU - Lathrop, Sarah L.

AU - Ye, Dongmei

AU - Ma, Thomas Y.

AU - Cerilli, Lisa A.

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The sequence of molecular changes leading to neoplastic transformation in the gallbladder remains elusive. The aim of this study is to characterize the spectrum of nuclear p16 protein product immunohistochemical expression in tissue microarray cores taken from resected gallbladders, comprising histologically normal gallbladder epithelia (n = 29), dyplastic epithelia (n = 19), reactive atypia (n = 7), and gallbladder adenocarcinoma (n = 23). Nuclear staining for p16 was evaluated for intensity (range, 0-3) and distribution (range, 0-3), and a summary staining score (range, 0-6) was obtained. Pairwise comparisons were significant for increased p16 protein expression in dysplastic epithelium (6/19, 31.6%) and invasive carcinoma (9/23, 39.1%) when compared to normal epithelium (0/29, 0%) (P = .003 and P = .0006, respectively). A quantitative increase of nuclear expression of p16 in reactive atypia (1/7, 14%) when compared to normal was also statistically significant (P = .049). Our data demonstrate that nuclear p16 expression is absent in normal gallbladder epithelium and is a frequent event in high-grade dysplasia of the gallbladder and gallbladder adenocarcinoma.

AB - The sequence of molecular changes leading to neoplastic transformation in the gallbladder remains elusive. The aim of this study is to characterize the spectrum of nuclear p16 protein product immunohistochemical expression in tissue microarray cores taken from resected gallbladders, comprising histologically normal gallbladder epithelia (n = 29), dyplastic epithelia (n = 19), reactive atypia (n = 7), and gallbladder adenocarcinoma (n = 23). Nuclear staining for p16 was evaluated for intensity (range, 0-3) and distribution (range, 0-3), and a summary staining score (range, 0-6) was obtained. Pairwise comparisons were significant for increased p16 protein expression in dysplastic epithelium (6/19, 31.6%) and invasive carcinoma (9/23, 39.1%) when compared to normal epithelium (0/29, 0%) (P = .003 and P = .0006, respectively). A quantitative increase of nuclear expression of p16 in reactive atypia (1/7, 14%) when compared to normal was also statistically significant (P = .049). Our data demonstrate that nuclear p16 expression is absent in normal gallbladder epithelium and is a frequent event in high-grade dysplasia of the gallbladder and gallbladder adenocarcinoma.

UR - http://www.scopus.com/inward/record.url?scp=43449123303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43449123303&partnerID=8YFLogxK

U2 - 10.1016/j.anndiagpath.2007.06.003

DO - 10.1016/j.anndiagpath.2007.06.003

M3 - Article

C2 - 18486890

AN - SCOPUS:43449123303

VL - 12

SP - 161

EP - 164

JO - Annals of Diagnostic Pathology

JF - Annals of Diagnostic Pathology

SN - 1092-9134

IS - 3

ER -