Expression of viral T-antigen in pathological tissues from transgenic mice carrying JC-SV40 chimeric DNAs

Holly G. Ressetar, Om Prakash, Richard John Frisque, Henry deF. Webster, Richard N. Re, Gerald L. Stoner

Research output: Contribution to journalArticle

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Abstract

Immunostaining methods were used to detect viral T-antigen and the cellular protein p53 in pathological tissues obtained from transgenic mice carrying JC-SV40 hybrid viral DNAs. A transgenic mouse carrying the SV40 regulatory region and JC virus (JCV) T-antigen-coding sequences exhibited an SV40-characteristic choroid plexus papilloma that expressed JCV T-antigen and p53. JCV-associated pathology was observed in two other mice in which the JCV regulatory signals directed SV40 T-antigen-induced adrenal neuroblastomas and brain neoplastic cells. However, these mice also exhibited an SV40-characteristic osteosarcoma and abdominal lymphoma that contained SV40 T-antigen and p53-positive cells. Contrasting thymic pathology was observed in the two types of mice where the SV40 regulatory region directed a JCV T-antigen-induced thymoma in one mouse, and the JCV regulatory region directed SV40 T-antigen-induced thymic hypoplasia in two other mice.

Original languageEnglish (US)
Pages (from-to)59-79
Number of pages21
JournalMolecular and Chemical Neuropathology
Volume20
Issue number1
DOIs
StatePublished - Sep 1 1993

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JC Virus
Viral Tumor Antigens
Transgenic Mice
Polyomavirus Transforming Antigens
Nucleic Acid Regulatory Sequences
DNA
Choroid Plexus Papilloma
Pathology
Thymoma
Viral DNA
Osteosarcoma
Neuroblastoma
Lymphoma
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology

Cite this

Ressetar, Holly G. ; Prakash, Om ; Frisque, Richard John ; deF. Webster, Henry ; Re, Richard N. ; Stoner, Gerald L. / Expression of viral T-antigen in pathological tissues from transgenic mice carrying JC-SV40 chimeric DNAs. In: Molecular and Chemical Neuropathology. 1993 ; Vol. 20, No. 1. pp. 59-79.
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Expression of viral T-antigen in pathological tissues from transgenic mice carrying JC-SV40 chimeric DNAs. / Ressetar, Holly G.; Prakash, Om; Frisque, Richard John; deF. Webster, Henry; Re, Richard N.; Stoner, Gerald L.

In: Molecular and Chemical Neuropathology, Vol. 20, No. 1, 01.09.1993, p. 59-79.

Research output: Contribution to journalArticle

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