Expression patterns in soybean resistant to Phakopsora pachyrhizi reveal the importance of peroxidases and lipoxygenases

Jane J. Choi, N. W. Alkharouf, Katherine T. Schneider, Ben F. Matthews, Reid D. Frederick

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Soybean rust caused by Phakopsora pachyrhizi Sydow is a devastating foliar disease that has spread to most soybean growing regions throughout the world, including the USA. Four independent rust resistance genes, Rpp1-Rpp4, have been identified in soybean that recognize specific isolates of P. pachyrhizi. A suppressive subtraction hybridization (SSH) complementary DNA (cDNA) library was constructed from the soybean accession PI200492, which contains Rpp1, after inoculation with two different isolates of P. pachyrhizi that result in susceptible or immune reactions. Both forward and reverse SSH were performed using cDNA from messenger RNA pooled from 1, 6, 12, 24, and 48 h post-inoculation. A total of 1,728 SSH clones were sequenced and compared to sequences in GenBank for similarity. Microarray analyses were conducted on a custom 7883 soybean-cDNA clone array encompassing all of the soybean-rust SSH clones and expressed sequence tags from four other soybean cDNA libraries. Results of the microarray revealed 558 cDNA clones differentially expressed in the immune reaction. The majority of the upregulated cDNA clones fell into the functional category of defense. In particular, cDNA clones with similarity to peroxidases and lipoxygenases were prevalent. Downregulated cDNA clones included those with similarity to cell-wall-associated protein, such as extensins, proline-rich proteins, and xyloglucan endotransglycosylases.

Original languageEnglish (US)
Pages (from-to)341-359
Number of pages19
JournalFunctional and Integrative Genomics
Volume8
Issue number4
DOIs
StatePublished - Apr 16 2008

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Lipoxygenases
Peroxidases
Soybeans
Complementary DNA
Clone Cells
Gene Library
Phakopsora pachyrhizi
Expressed Sequence Tags
Nucleic Acid Databases
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Proline
Cell Wall
Proteins
Down-Regulation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Choi, Jane J. ; Alkharouf, N. W. ; Schneider, Katherine T. ; Matthews, Ben F. ; Frederick, Reid D. / Expression patterns in soybean resistant to Phakopsora pachyrhizi reveal the importance of peroxidases and lipoxygenases. In: Functional and Integrative Genomics. 2008 ; Vol. 8, No. 4. pp. 341-359.
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Expression patterns in soybean resistant to Phakopsora pachyrhizi reveal the importance of peroxidases and lipoxygenases. / Choi, Jane J.; Alkharouf, N. W.; Schneider, Katherine T.; Matthews, Ben F.; Frederick, Reid D.

In: Functional and Integrative Genomics, Vol. 8, No. 4, 16.04.2008, p. 341-359.

Research output: Contribution to journalArticle

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