Expression patterns of the transcription factor AP-2α during hair follicle morphogenesis and cycling

Andrey A. Panteleyev, Pamela J. Mitchell, Ralf Paus, Angela M. Christiano

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

AP-2α is a member of a family of transcription factors expressed in cells of the epithelial and neural crest lineage. AP-2α plays an essential role in embryonic development and in regulation of epithelial gene transcription. To further characterize the role of AP-2α in skin biology, we assessed its expression in the skin of C57BL/6J mice during defined stages of hair follicle morphogenesis and cycling. During early hair follicle morphogenesis, AP-2α was upregulated in the epidermal placode, in the basal keratinocytes of the hair follicle bud, and then in the inner root sheath. The follicular papilla cells underwent a brief upregulation of AP-2α expression during the initiation of hair shaft formation and active hair follicle downward growth. Completion of hair follicle morphogenesis was associated with a marked reduction of AP-2α immunoreactivity in the lower portion of the hair follicle including both epithelial and mesenchymal compartments. In adolescent mouse skin, consistently strong AP-2α expression was found in the basal keratinocytes of the epidermis, in the hair follicle infundibulum, and in the sebocytes. In the follicular papilla, AP-2α was weakly expressed in telogen, significantly upregulated in early anagen, then gradually declined, and reappeared again in middle catagen. In the inner root sheaths, AP-2α expression was detected during early and middle anagen and during middle catagen stages. Prominent AP-2α expression was also seen in the zone of club hair formation. Therefore, AP-2α upregulation in both epithelial and mesenchymal hair follicle compartments was coordinated with initiation of major remodeling processes. Our findings support the use of the hair follicle as a model to explore the role of AP-2α in physiologic remodeling of developing organs and in reciprocal ectodermal-mesenchymal interactions.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalJournal of Investigative Dermatology
Volume121
Issue number1
DOIs
StatePublished - Jul 1 2003

Fingerprint

Transcription Factor AP-2
Hair Follicle
Morphogenesis
Skin
Transcription
Transcription Factors
Genes
Keratinocytes
Hair
Up-Regulation
Neural Crest
Pituitary Gland
Inbred C57BL Mouse
Epidermis
Embryonic Development

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Panteleyev, Andrey A. ; Mitchell, Pamela J. ; Paus, Ralf ; Christiano, Angela M. / Expression patterns of the transcription factor AP-2α during hair follicle morphogenesis and cycling. In: Journal of Investigative Dermatology. 2003 ; Vol. 121, No. 1. pp. 13-19.
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abstract = "AP-2α is a member of a family of transcription factors expressed in cells of the epithelial and neural crest lineage. AP-2α plays an essential role in embryonic development and in regulation of epithelial gene transcription. To further characterize the role of AP-2α in skin biology, we assessed its expression in the skin of C57BL/6J mice during defined stages of hair follicle morphogenesis and cycling. During early hair follicle morphogenesis, AP-2α was upregulated in the epidermal placode, in the basal keratinocytes of the hair follicle bud, and then in the inner root sheath. The follicular papilla cells underwent a brief upregulation of AP-2α expression during the initiation of hair shaft formation and active hair follicle downward growth. Completion of hair follicle morphogenesis was associated with a marked reduction of AP-2α immunoreactivity in the lower portion of the hair follicle including both epithelial and mesenchymal compartments. In adolescent mouse skin, consistently strong AP-2α expression was found in the basal keratinocytes of the epidermis, in the hair follicle infundibulum, and in the sebocytes. In the follicular papilla, AP-2α was weakly expressed in telogen, significantly upregulated in early anagen, then gradually declined, and reappeared again in middle catagen. In the inner root sheaths, AP-2α expression was detected during early and middle anagen and during middle catagen stages. Prominent AP-2α expression was also seen in the zone of club hair formation. Therefore, AP-2α upregulation in both epithelial and mesenchymal hair follicle compartments was coordinated with initiation of major remodeling processes. Our findings support the use of the hair follicle as a model to explore the role of AP-2α in physiologic remodeling of developing organs and in reciprocal ectodermal-mesenchymal interactions.",
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Expression patterns of the transcription factor AP-2α during hair follicle morphogenesis and cycling. / Panteleyev, Andrey A.; Mitchell, Pamela J.; Paus, Ralf; Christiano, Angela M.

In: Journal of Investigative Dermatology, Vol. 121, No. 1, 01.07.2003, p. 13-19.

Research output: Contribution to journalArticle

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N2 - AP-2α is a member of a family of transcription factors expressed in cells of the epithelial and neural crest lineage. AP-2α plays an essential role in embryonic development and in regulation of epithelial gene transcription. To further characterize the role of AP-2α in skin biology, we assessed its expression in the skin of C57BL/6J mice during defined stages of hair follicle morphogenesis and cycling. During early hair follicle morphogenesis, AP-2α was upregulated in the epidermal placode, in the basal keratinocytes of the hair follicle bud, and then in the inner root sheath. The follicular papilla cells underwent a brief upregulation of AP-2α expression during the initiation of hair shaft formation and active hair follicle downward growth. Completion of hair follicle morphogenesis was associated with a marked reduction of AP-2α immunoreactivity in the lower portion of the hair follicle including both epithelial and mesenchymal compartments. In adolescent mouse skin, consistently strong AP-2α expression was found in the basal keratinocytes of the epidermis, in the hair follicle infundibulum, and in the sebocytes. In the follicular papilla, AP-2α was weakly expressed in telogen, significantly upregulated in early anagen, then gradually declined, and reappeared again in middle catagen. In the inner root sheaths, AP-2α expression was detected during early and middle anagen and during middle catagen stages. Prominent AP-2α expression was also seen in the zone of club hair formation. Therefore, AP-2α upregulation in both epithelial and mesenchymal hair follicle compartments was coordinated with initiation of major remodeling processes. Our findings support the use of the hair follicle as a model to explore the role of AP-2α in physiologic remodeling of developing organs and in reciprocal ectodermal-mesenchymal interactions.

AB - AP-2α is a member of a family of transcription factors expressed in cells of the epithelial and neural crest lineage. AP-2α plays an essential role in embryonic development and in regulation of epithelial gene transcription. To further characterize the role of AP-2α in skin biology, we assessed its expression in the skin of C57BL/6J mice during defined stages of hair follicle morphogenesis and cycling. During early hair follicle morphogenesis, AP-2α was upregulated in the epidermal placode, in the basal keratinocytes of the hair follicle bud, and then in the inner root sheath. The follicular papilla cells underwent a brief upregulation of AP-2α expression during the initiation of hair shaft formation and active hair follicle downward growth. Completion of hair follicle morphogenesis was associated with a marked reduction of AP-2α immunoreactivity in the lower portion of the hair follicle including both epithelial and mesenchymal compartments. In adolescent mouse skin, consistently strong AP-2α expression was found in the basal keratinocytes of the epidermis, in the hair follicle infundibulum, and in the sebocytes. In the follicular papilla, AP-2α was weakly expressed in telogen, significantly upregulated in early anagen, then gradually declined, and reappeared again in middle catagen. In the inner root sheaths, AP-2α expression was detected during early and middle anagen and during middle catagen stages. Prominent AP-2α expression was also seen in the zone of club hair formation. Therefore, AP-2α upregulation in both epithelial and mesenchymal hair follicle compartments was coordinated with initiation of major remodeling processes. Our findings support the use of the hair follicle as a model to explore the role of AP-2α in physiologic remodeling of developing organs and in reciprocal ectodermal-mesenchymal interactions.

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