Expression strategy of aedes albopictus densovirus

Hanh T. Pham, Françoise Xavière Jousset, Jonathan Perreault, Hiroko Shike, Jozsef Szelei, Max Bergoin, Peter Tijssen

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.

Original languageEnglish (US)
Pages (from-to)9928-9932
Number of pages5
JournalJournal of virology
Volume87
Issue number17
DOIs
StatePublished - 2013

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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