Expression strategy of aedes albopictus densovirus

Hanh T. Pham, Françoise Xavière Jousset, Jonathan Perreault, Hiroko Shike, Jozsef Szelei, Max Bergoin, Peter Tijssen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.

Original languageEnglish (US)
Pages (from-to)9928-9932
Number of pages5
JournalJournal of virology
Volume87
Issue number17
DOIs
StatePublished - Sep 5 2013

Fingerprint

Aedes albopictus densovirus
Densovirus
Aedes
Brevidensovirus
transcription (genetics)
promoter regions
Messenger RNA
start codon
Densovirinae
human cell lines
rapid amplification of cDNA ends
assays
luciferase
Northern blotting
Viral Structural Proteins
Initiator Codon
Transcription Initiation Site
nucleotides
Ribonucleases
Luciferases

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Pham, H. T., Jousset, F. X., Perreault, J., Shike, H., Szelei, J., Bergoin, M., & Tijssen, P. (2013). Expression strategy of aedes albopictus densovirus. Journal of virology, 87(17), 9928-9932. https://doi.org/10.1128/JVI.01259-13
Pham, Hanh T. ; Jousset, Françoise Xavière ; Perreault, Jonathan ; Shike, Hiroko ; Szelei, Jozsef ; Bergoin, Max ; Tijssen, Peter. / Expression strategy of aedes albopictus densovirus. In: Journal of virology. 2013 ; Vol. 87, No. 17. pp. 9928-9932.
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Pham, HT, Jousset, FX, Perreault, J, Shike, H, Szelei, J, Bergoin, M & Tijssen, P 2013, 'Expression strategy of aedes albopictus densovirus', Journal of virology, vol. 87, no. 17, pp. 9928-9932. https://doi.org/10.1128/JVI.01259-13

Expression strategy of aedes albopictus densovirus. / Pham, Hanh T.; Jousset, Françoise Xavière; Perreault, Jonathan; Shike, Hiroko; Szelei, Jozsef; Bergoin, Max; Tijssen, Peter.

In: Journal of virology, Vol. 87, No. 17, 05.09.2013, p. 9928-9932.

Research output: Contribution to journalArticle

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Pham HT, Jousset FX, Perreault J, Shike H, Szelei J, Bergoin M et al. Expression strategy of aedes albopictus densovirus. Journal of virology. 2013 Sep 5;87(17):9928-9932. https://doi.org/10.1128/JVI.01259-13