TY - JOUR
T1 - Expression strategy of aedes albopictus densovirus
AU - Pham, Hanh T.
AU - Jousset, Françoise Xavière
AU - Perreault, Jonathan
AU - Shike, Hiroko
AU - Szelei, Jozsef
AU - Bergoin, Max
AU - Tijssen, Peter
PY - 2013
Y1 - 2013
N2 - The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.
AB - The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.
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U2 - 10.1128/JVI.01259-13
DO - 10.1128/JVI.01259-13
M3 - Article
C2 - 23804640
AN - SCOPUS:84883282640
SN - 0022-538X
VL - 87
SP - 9928
EP - 9932
JO - Journal of Virology
JF - Journal of Virology
IS - 17
ER -