Extensive rearrangement of the arabidopsis mitochondrial genome elicits cellular conditions for thermotolerance

Vikas Shedge, Jaime Davila, Maria P. Arrieta-Montiel, Saleem Mohammed, Sally A. Mackenzie

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Three nuclear genes involved in plant mitochondrial recombination surveillance have been previously identified. Simultaneous disruption of two of these genes, MutS Homolog1 (MSH1) and RECA3, results in extensive rearrangement of the mitochondrial genome and dramatic changes in plant growth. We have capitalized on these changes in mitochondrial genome organization to understand the role mitochondria play in plant cellular and developmental processes. Transcript profiling of the double mutants grown under normal conditions revealed differential regulation of numerous nuclear genes involved in stress responses together with increased levels of polyadenylated mitochondrial transcripts. We show that extensive rearrangement of the mitochondrial genome in Arabidopsis (Arabidopsis thaliana) directly elicits physiological stress responses in plants, with msh1 recA3 double mutants exhibiting enhanced thermotolerance. Likewise, we show that mitochondrial transcriptional changes are associated with genome recombination, so that differential gene modulation is accomplished, at least in part, through altered gene copy number.

Original languageEnglish (US)
Pages (from-to)1960-1970
Number of pages11
JournalPlant physiology
Volume152
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Mitochondrial Genome
heat tolerance
Arabidopsis
Genetic Recombination
Genes
stress response
genes
gene dosage
mutants
Physiological Stress
Gene Dosage
Mitochondria
mitochondria
Arabidopsis thaliana
plant growth
Genome
genome
Thermotolerance
mitochondrial genome
monitoring

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Shedge, Vikas ; Davila, Jaime ; Arrieta-Montiel, Maria P. ; Mohammed, Saleem ; Mackenzie, Sally A. / Extensive rearrangement of the arabidopsis mitochondrial genome elicits cellular conditions for thermotolerance. In: Plant physiology. 2010 ; Vol. 152, No. 4. pp. 1960-1970.
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Extensive rearrangement of the arabidopsis mitochondrial genome elicits cellular conditions for thermotolerance. / Shedge, Vikas; Davila, Jaime; Arrieta-Montiel, Maria P.; Mohammed, Saleem; Mackenzie, Sally A.

In: Plant physiology, Vol. 152, No. 4, 01.04.2010, p. 1960-1970.

Research output: Contribution to journalArticle

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