Extraction and quantification of nicardipine in human plasma

Qing Cheng Meng, Albert T. Cheung, Dmitri Guvakov, Stuart J. Weiss, Joseph S. Savino, Ivan S. Salgo, Bryan E. Marshall

Research output: Contribution to journalArticle

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Abstract

A novel simple method of extraction, separation, identification and quantification of nicardipine in human plasma samples was completely studied. The human plasma samples were initially purified by solid-phase extraction (SPE) using a C 18 cartridge. The extracted samples were separated and nicardipine present in the samples was quantified by high-performance liquid chromatography (HPLC) on a reversed-phase C 18 column employing a mobile phase consisting of 60% (v/v) acetonitrile in 0.02 M NaH 2 PO 4 with pH of 6.3 and a variable wavelength UV detector set at 254 nm. The recovery of nicardipine from plasma samples using selective SPE was 91±6.0% and had less interfering compounds in the HPLC analysis compared to the use of liquid-liquid (L/L) extraction. In the HPLC analysis, examining the effect of pH values of the mobile phase on the capacity factor (k') of nicardipine revealed a method for selecting a critical k' value of nicardipine to eliminate interfering peaks near the peak specific to the analyte. This method for quantification of nicardipine in human plasma samples was suitable for studying the pharmacokinetic profile of nicardipine administered as an intravenous bolus to cardiac surgical patients. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalJournal of Chromatography B: Biomedical Applications
Volume718
Issue number1
DOIs
StatePublished - Oct 23 1998

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Plasma (human)
Nicardipine
High performance liquid chromatography
Ultraviolet detectors
Pharmacokinetics
Liquids
Plasmas
Recovery
Wavelength

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Meng, Q. C., Cheung, A. T., Guvakov, D., Weiss, S. J., Savino, J. S., Salgo, I. S., & Marshall, B. E. (1998). Extraction and quantification of nicardipine in human plasma. Journal of Chromatography B: Biomedical Applications, 718(1), 121-127. https://doi.org/10.1016/S0378-4347(98)00352-1
Meng, Qing Cheng ; Cheung, Albert T. ; Guvakov, Dmitri ; Weiss, Stuart J. ; Savino, Joseph S. ; Salgo, Ivan S. ; Marshall, Bryan E. / Extraction and quantification of nicardipine in human plasma. In: Journal of Chromatography B: Biomedical Applications. 1998 ; Vol. 718, No. 1. pp. 121-127.
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Extraction and quantification of nicardipine in human plasma. / Meng, Qing Cheng; Cheung, Albert T.; Guvakov, Dmitri; Weiss, Stuart J.; Savino, Joseph S.; Salgo, Ivan S.; Marshall, Bryan E.

In: Journal of Chromatography B: Biomedical Applications, Vol. 718, No. 1, 23.10.1998, p. 121-127.

Research output: Contribution to journalArticle

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