Extraction, evaluation, and amplification of DNA from decalcified and undecalcified United States Civil War bone

D. L. Fisher, Mitchell Mark Holland, L. Mitchell, P. S. Sledzik, A. W. Wilcox, M. Wadhams, V. W. Weedn

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Deoxyribonucleic acid (DNA) was extracted from documented skeletal specimens of U.S. Civil War soldiers to determine the need for decalcification prior to extraction. The polymerase chain reaction (PCR) was performed to determine if the calcification state had an effect on the ability to amplify the extracts and to determine how successful amplification would be with these aged specimens. Bone samples were pulverized to a fine powder and divided into two sets. One set of samples was decalcified and the other set left undecalcified. Both sets were extracted using an organic procedure. The results demonstrate that decalcification is not a necessary step in the extraction process and that the yield of DNA is generally two times greater when decalcification is omitted. Furthermore, the calcification state had no effect on the ability to perform the PCR. Although the extracted DNA was very degraded, a 410 base pair (bp) segment of the mitochondrial DNA (mtDNA) control region was amplified. These results suggest that DNA can be extracted and amplified from 125 year old bone without decalcification, which may assist in the identity of modern and historic forensic specimens.

Original languageEnglish (US)
Pages (from-to)60-68
Number of pages9
JournalJournal of Forensic Sciences
Volume38
Issue number1
StatePublished - Jan 1 1993

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Bone and Bones
DNA
Polymerase Chain Reaction
Military Personnel
Base Pairing
Powders
Warfare

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Genetics

Cite this

Fisher, D. L., Holland, M. M., Mitchell, L., Sledzik, P. S., Wilcox, A. W., Wadhams, M., & Weedn, V. W. (1993). Extraction, evaluation, and amplification of DNA from decalcified and undecalcified United States Civil War bone. Journal of Forensic Sciences, 38(1), 60-68.
Fisher, D. L. ; Holland, Mitchell Mark ; Mitchell, L. ; Sledzik, P. S. ; Wilcox, A. W. ; Wadhams, M. ; Weedn, V. W. / Extraction, evaluation, and amplification of DNA from decalcified and undecalcified United States Civil War bone. In: Journal of Forensic Sciences. 1993 ; Vol. 38, No. 1. pp. 60-68.
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Fisher, DL, Holland, MM, Mitchell, L, Sledzik, PS, Wilcox, AW, Wadhams, M & Weedn, VW 1993, 'Extraction, evaluation, and amplification of DNA from decalcified and undecalcified United States Civil War bone', Journal of Forensic Sciences, vol. 38, no. 1, pp. 60-68.

Extraction, evaluation, and amplification of DNA from decalcified and undecalcified United States Civil War bone. / Fisher, D. L.; Holland, Mitchell Mark; Mitchell, L.; Sledzik, P. S.; Wilcox, A. W.; Wadhams, M.; Weedn, V. W.

In: Journal of Forensic Sciences, Vol. 38, No. 1, 01.01.1993, p. 60-68.

Research output: Contribution to journalArticle

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