Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis

Kellen L. Olszewski, Manuel Llinás

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Metabolomics is an increasingly common analytical approach for investigating metabolic networks of pathogenic organisms. This may be of particular use in the study of parasitic infections due to the intrinsic metabolic connection between the parasite and its host. In vitro cultures of the malaria parasite Plasmodium falciparum present a valuable platform to elucidate the structure and dynamics of the parasite's metabolic network and to determine the mechanisms of action of antimalarial drugs and drug resistance mutations. Accurately measuring metabolite levels requires a reproducible method for quantifying intracellular metabolites. Here we present a simple protocol for extracting hydrophilic metabolites from P. falciparum -infected erythrocyte cultures.

Original languageEnglish (US)
Title of host publicationMalaria
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages259-266
Number of pages8
ISBN (Print)9781627030250
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume923
ISSN (Print)1064-3745

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Olszewski, K. L., & Llinás, M. (2013). Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis. In Malaria: Methods and Protocols (pp. 259-266). (Methods in Molecular Biology; Vol. 923). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-26-7_17