Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis

Kellen L. Olszewski, Manuel Llinas

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Metabolomics is an increasingly common analytical approach for investigating metabolic networks of pathogenic organisms. This may be of particular use in the study of parasitic infections due to the intrinsic metabolic connection between the parasite and its host. In vitro cultures of the malaria parasite Plasmodium falciparum present a valuable platform to elucidate the structure and dynamics of the parasite's metabolic network and to determine the mechanisms of action of antimalarial drugs and drug resistance mutations. Accurately measuring metabolite levels requires a reproducible method for quantifying intracellular metabolites. Here we present a simple protocol for extracting hydrophilic metabolites from P. falciparum -infected erythrocyte cultures.

Original languageEnglish (US)
Title of host publicationMalaria
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages259-266
Number of pages8
ISBN (Print)9781627030250
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume923
ISSN (Print)1064-3745

Fingerprint

Metabolomics
Plasmodium falciparum
Parasites
Erythrocytes
Metabolic Networks and Pathways
Parasitic Diseases
Falciparum Malaria
Antimalarials
Drug Resistance
Mutation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Olszewski, K. L., & Llinas, M. (2013). Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis. In Malaria: Methods and Protocols (pp. 259-266). (Methods in Molecular Biology; Vol. 923). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-26-7_17
Olszewski, Kellen L. ; Llinas, Manuel. / Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis. Malaria: Methods and Protocols. Humana Press Inc., 2013. pp. 259-266 (Methods in Molecular Biology).
@inbook{830f8f7160c34904afb403c458079152,
title = "Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis",
abstract = "Metabolomics is an increasingly common analytical approach for investigating metabolic networks of pathogenic organisms. This may be of particular use in the study of parasitic infections due to the intrinsic metabolic connection between the parasite and its host. In vitro cultures of the malaria parasite Plasmodium falciparum present a valuable platform to elucidate the structure and dynamics of the parasite's metabolic network and to determine the mechanisms of action of antimalarial drugs and drug resistance mutations. Accurately measuring metabolite levels requires a reproducible method for quantifying intracellular metabolites. Here we present a simple protocol for extracting hydrophilic metabolites from P. falciparum -infected erythrocyte cultures.",
author = "Olszewski, {Kellen L.} and Manuel Llinas",
year = "2013",
month = "1",
day = "1",
doi = "10.1007/978-1-62703-26-7_17",
language = "English (US)",
isbn = "9781627030250",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "259--266",
booktitle = "Malaria",

}

Olszewski, KL & Llinas, M 2013, Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis. in Malaria: Methods and Protocols. Methods in Molecular Biology, vol. 923, Humana Press Inc., pp. 259-266. https://doi.org/10.1007/978-1-62703-26-7_17

Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis. / Olszewski, Kellen L.; Llinas, Manuel.

Malaria: Methods and Protocols. Humana Press Inc., 2013. p. 259-266 (Methods in Molecular Biology; Vol. 923).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis

AU - Olszewski, Kellen L.

AU - Llinas, Manuel

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Metabolomics is an increasingly common analytical approach for investigating metabolic networks of pathogenic organisms. This may be of particular use in the study of parasitic infections due to the intrinsic metabolic connection between the parasite and its host. In vitro cultures of the malaria parasite Plasmodium falciparum present a valuable platform to elucidate the structure and dynamics of the parasite's metabolic network and to determine the mechanisms of action of antimalarial drugs and drug resistance mutations. Accurately measuring metabolite levels requires a reproducible method for quantifying intracellular metabolites. Here we present a simple protocol for extracting hydrophilic metabolites from P. falciparum -infected erythrocyte cultures.

AB - Metabolomics is an increasingly common analytical approach for investigating metabolic networks of pathogenic organisms. This may be of particular use in the study of parasitic infections due to the intrinsic metabolic connection between the parasite and its host. In vitro cultures of the malaria parasite Plasmodium falciparum present a valuable platform to elucidate the structure and dynamics of the parasite's metabolic network and to determine the mechanisms of action of antimalarial drugs and drug resistance mutations. Accurately measuring metabolite levels requires a reproducible method for quantifying intracellular metabolites. Here we present a simple protocol for extracting hydrophilic metabolites from P. falciparum -infected erythrocyte cultures.

UR - http://www.scopus.com/inward/record.url?scp=84934434264&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84934434264&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-26-7_17

DO - 10.1007/978-1-62703-26-7_17

M3 - Chapter

C2 - 22990783

AN - SCOPUS:84934434264

SN - 9781627030250

T3 - Methods in Molecular Biology

SP - 259

EP - 266

BT - Malaria

PB - Humana Press Inc.

ER -

Olszewski KL, Llinas M. Extraction of hydrophilic metabolites from plasmodium falciparum -infected erythrocytes for metabolomic analysis. In Malaria: Methods and Protocols. Humana Press Inc. 2013. p. 259-266. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-26-7_17