Fabrication of phospholipid bilayer-coated microchannels for on-chip immunoassays

T. Yang, S. Y. Jung, H. Mao, P. S. Cremer

Research output: Contribution to journalArticle

234 Citations (Scopus)

Abstract

Herein we describe a new class of microfluidic immunoassays based upon solid supported lipid bilayers. Two-dimensionally fluid bilayer material, which can accommodate multivalent binding between surface-bound ligands and aqueous receptors, was coated on the surface of poly-(dimethylsiloxane) microchannels. The bilayers contained dinitrophenyl (DNP)-conjugated lipids for binding with bivalent anti-DNP antibodies. Twelve independent data points of surface coverage versus bulk protein concentration could be made simultaneously by forming a linear array of channels and flowing fluorescently labeled antibodies into them. This enabled an entire binding curve to be obtained in a single experiment. The measured apparent binding constant for the DNP/anti-DNP system was 1.8 μM The methodology for performing heterogeneous assays developed here not only produces rapid results but also requires much less protein than traditional procedures and eliminates some standard sources of experimental error.

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalAnalytical chemistry
Volume73
Issue number2
DOIs
StatePublished - Jan 15 2001

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Microchannels
Phospholipids
Fabrication
Lipid bilayers
Antibodies
Microfluidics
Assays
Proteins
Ligands
Lipids
Fluids
Experiments

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

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Fabrication of phospholipid bilayer-coated microchannels for on-chip immunoassays. / Yang, T.; Jung, S. Y.; Mao, H.; Cremer, P. S.

In: Analytical chemistry, Vol. 73, No. 2, 15.01.2001, p. 165-169.

Research output: Contribution to journalArticle

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