Fabrication process of microsurgical tools for single-cell trapping and intracytoplasmic injection

Pak Kin Wong, Umberto Ulmanella, Chih Ming Ho

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In this paper, we introduce chemical etching-based processes for fabricating micromanipulators from fused silica microcapillary tubing. The resulting devices are capable of manipulating individual cells and can be used for intracytoplasmic injection. Two types of micromanipulating tools: single-cell trappers and micro-injectors, were fabricated with two single-step etching processes. These etching mechanisms, which are either surface tension controlled or diffusion rate limited, are discussed in detail with experimental verifications. The fabrication processes are reproducible and amenable to mass production due to their simplicity and low fabrication cost. Single cell capture and intracytoplasmic injection have been successfully demonstrated on brassica oleracea protoplasts.

Original languageEnglish (US)
Pages (from-to)940-946
Number of pages7
JournalJournal of Microelectromechanical Systems
Volume13
Issue number6
DOIs
StatePublished - Dec 1 2004

Fingerprint

Etching
Fabrication
Micromanipulators
Tubing
Fused silica
Surface tension
Costs

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

@article{d8cd4cdee97c498c8d09e74d578025b7,
title = "Fabrication process of microsurgical tools for single-cell trapping and intracytoplasmic injection",
abstract = "In this paper, we introduce chemical etching-based processes for fabricating micromanipulators from fused silica microcapillary tubing. The resulting devices are capable of manipulating individual cells and can be used for intracytoplasmic injection. Two types of micromanipulating tools: single-cell trappers and micro-injectors, were fabricated with two single-step etching processes. These etching mechanisms, which are either surface tension controlled or diffusion rate limited, are discussed in detail with experimental verifications. The fabrication processes are reproducible and amenable to mass production due to their simplicity and low fabrication cost. Single cell capture and intracytoplasmic injection have been successfully demonstrated on brassica oleracea protoplasts.",
author = "Wong, {Pak Kin} and Umberto Ulmanella and Ho, {Chih Ming}",
year = "2004",
month = "12",
day = "1",
doi = "10.1109/JMEMS.2004.838382",
language = "English (US)",
volume = "13",
pages = "940--946",
journal = "Journal of Microelectromechanical Systems",
issn = "1057-7157",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "6",

}

Fabrication process of microsurgical tools for single-cell trapping and intracytoplasmic injection. / Wong, Pak Kin; Ulmanella, Umberto; Ho, Chih Ming.

In: Journal of Microelectromechanical Systems, Vol. 13, No. 6, 01.12.2004, p. 940-946.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fabrication process of microsurgical tools for single-cell trapping and intracytoplasmic injection

AU - Wong, Pak Kin

AU - Ulmanella, Umberto

AU - Ho, Chih Ming

PY - 2004/12/1

Y1 - 2004/12/1

N2 - In this paper, we introduce chemical etching-based processes for fabricating micromanipulators from fused silica microcapillary tubing. The resulting devices are capable of manipulating individual cells and can be used for intracytoplasmic injection. Two types of micromanipulating tools: single-cell trappers and micro-injectors, were fabricated with two single-step etching processes. These etching mechanisms, which are either surface tension controlled or diffusion rate limited, are discussed in detail with experimental verifications. The fabrication processes are reproducible and amenable to mass production due to their simplicity and low fabrication cost. Single cell capture and intracytoplasmic injection have been successfully demonstrated on brassica oleracea protoplasts.

AB - In this paper, we introduce chemical etching-based processes for fabricating micromanipulators from fused silica microcapillary tubing. The resulting devices are capable of manipulating individual cells and can be used for intracytoplasmic injection. Two types of micromanipulating tools: single-cell trappers and micro-injectors, were fabricated with two single-step etching processes. These etching mechanisms, which are either surface tension controlled or diffusion rate limited, are discussed in detail with experimental verifications. The fabrication processes are reproducible and amenable to mass production due to their simplicity and low fabrication cost. Single cell capture and intracytoplasmic injection have been successfully demonstrated on brassica oleracea protoplasts.

UR - http://www.scopus.com/inward/record.url?scp=10944221201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10944221201&partnerID=8YFLogxK

U2 - 10.1109/JMEMS.2004.838382

DO - 10.1109/JMEMS.2004.838382

M3 - Article

AN - SCOPUS:10944221201

VL - 13

SP - 940

EP - 946

JO - Journal of Microelectromechanical Systems

JF - Journal of Microelectromechanical Systems

SN - 1057-7157

IS - 6

ER -