Facile synthesis of magnetic mesoporous hollow carbon microspheres for rapid capture of low-concentration peptides

Gong Cheng, Ming Da Zhou, Si Yang Zheng

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Mesoporous and hollow carbon microspheres embedded with magnetic nanoparticles (denoted as MHM) were prepared via a facile self-sacrificial method for rapid capture of low-abundant peptides from complex biological samples. The morphology, structure, surface property, and magnetism were well-characterized. The hollow magnetic carbon microspheres have a saturation magnetization value of 130.2 emu g-1 at room temperature and a Brunauer-Emmett-Teller specific surface area of 48.8 m2 g -1 with an average pore size of 9.2 nm for the mesoporous carbon shell. The effectiveness of these MHM affinity microspheres for capture of low-concentration peptides was evaluated by standard peptides, complex protein digests, and real biological samples. These multifunctional hollow carbon microspheres can realize rapid capture and convenient separation of low-concentration peptides. They were validated to have better performance than magnetic mesoporous silica and commercial peptide-enrichment products. In addition, they can be easily recycled and present excellent reusability. Therefore, it is expected that this work may provide a promising tool for high-throughput discovery of peptide biomarkers from biological samples for disease diagnosis and other biomedical applications.

Original languageEnglish (US)
Pages (from-to)12719-12728
Number of pages10
JournalACS Applied Materials and Interfaces
Volume6
Issue number15
DOIs
StatePublished - Aug 13 2014

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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