Factors affecting SP-A-mediated phagocytosis in human monocytic cell lines

Jianqiang Ding, Todd M. Umstead, Joanna Floros, David Phelps

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Surfactant protein-A enhances the phagocytosis and killing of many pathogens, although studying this effect in an assortment of models and different experimental protocols has sometimes yielded conflicting results. In this report, using the human THP-1 cell line as the primary phagocytic cell, we systematically examined several models where microspheres, Staphylococcus aureus and Escherichia coli were used for targets. We found that SP-A derived from human lavage appeared to enhance phagocytosis by two different mechanisms; by SP-A binding of the target to enhance its recognition and subsequent phagocytosis and by a direct SP-A stimulatory effect on the phagocyte itself. Both SP-A mechanisms occurred with different targets in the same experimental system and the SP-A effects were qualitatively (but not quantitatively) comparable in several human cell lines (THP-1, U937, Mono-Mac-6). We also found that the SP-A effects were abrogated when SP-A was combined with surfactant lipids, but the lipids did not affect the basal level of phagocytosis or phagocytosis by mechanisms not involving SP-A. Moreover, the stimulatory effect of SP-A was pH-dependent and appeared to be independent of several other phagocytic mechanisms, including those mediated by Fc receptors and mannose receptor.

Original languageEnglish (US)
Pages (from-to)637-650
Number of pages14
JournalRespiratory Medicine
Volume98
Issue number7
DOIs
StatePublished - Jul 2004

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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