Factors influencing pituitary glycogen metabolism and gonadotropic hormone release. I. Luteinizing hormone releasing hormone

T. Makino, L. M. Demers, R. O. Greep

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The mechanism of release of luteinizing hormone (LH) and follicle stimulating hormone (FSH) from the rat anterior pituitary by LH releasing hormone (LH RH) was further evaluated by studies on pituitary glycogen metabolism and its relation to the hormone release mechanism in vitro. Pituitary glycogen content and the activity levels of its 2 major regulatory enzymes, glycogen synthetase and glycogen phosphorylase, were analyzed after exposure to different doses of synthetic LH RH in vitro. Less than 5 ng of LH RH induced within minutes a maximum glycogenolytic response with an increase in the proportion of pituitary phosphorylase in the more active a form and a decrease in pituitary glycogen. Exogenous N6,O2' dibutyryl cyclic AMP (10 millimol) with theophylline (1 millimol) mimicked the effect of LH RH. 15 ng of LH RH also induced a significant release of LH and FSH into the medium during 4 hr of incubation. These results suggest that pituitary glycogen may be a source of the energy required for the hypothalamic hypophysiotropic hormone release mechanism.

Original languageEnglish (US)
Pages (from-to)872-877
Number of pages6
JournalFertility and sterility
Volume25
Issue number10
StatePublished - Jan 1 1974

Fingerprint

Luteinizing Hormone
Glycogen
Gonadotropin-Releasing Hormone
Hormones
Pituitary Hormone-Releasing Hormones
Follicle Stimulating Hormone
Anterior Pituitary Hormones
Glycogen Phosphorylase
Glycogen Synthase
Phosphorylases
Bucladesine
Theophylline
Enzymes

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

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Factors influencing pituitary glycogen metabolism and gonadotropic hormone release. I. Luteinizing hormone releasing hormone. / Makino, T.; Demers, L. M.; Greep, R. O.

In: Fertility and sterility, Vol. 25, No. 10, 01.01.1974, p. 872-877.

Research output: Contribution to journalArticle

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