Failure to Detect Bovine Viral Diarrhea Virus in Necropsied Farm-Raised White-Tailed Deer (Odocoileus Virginianus) in Pennsylvania

Jason W. Brooks, Douglas W. Key, Arthur L. Hattel, Ernest P. Hovingh, Ryan Peterson, Daniel P. Shaw, Jenny S. Fisher

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Between January 1 and December 31, 2005 gross and histologic examinations were performed on carcasses of 61 farm-raised white-tailed deer originating from Pennsylvania. Single-tube real-time reverse transcription polymerase chain reaction (real-time RT-PCR) for the detection of bovine viral diarrhea virus type 1 (BVDV-1) and type 2 (BVDV-2) was performed on each animal. Virus isolation was performed on tissue samples from 25 of 61 animals. Immunohistochemical (IHC) staining of ear-notch skin to identify BVDV antigen was performed on each animal. All tissues samples tested negative for both BVDV-1 and BVDV-2 by real-time RT-PCR, virus isolation, and IHC. Gross or histopathologic lesions suggestive of BVDV infection were not detected. Results of this study suggest that BVD is not a common cause of mortality in farm-raised white-tailed deer in Pennsylvania.

Original languageEnglish (US)
Pages (from-to)298-300
Number of pages3
JournalJournal of Veterinary Diagnostic Investigation
Volume19
Issue number3
DOIs
StatePublished - Dec 1 2007

Fingerprint

Bovine viral diarrhea virus 1
Bovine Viral Diarrhea Viruses
Deer
Bovine viral diarrhea virus
Bovine viral diarrhea virus 2
Odocoileus virginianus
reverse transcriptase polymerase chain reaction
farms
Real-Time Polymerase Chain Reaction
Viruses
viruses
animals
bovine viral diarrhea
skin (animal)
lesions (animal)
Reverse Transcription
Ear
ears
quantitative polymerase chain reaction
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

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title = "Failure to Detect Bovine Viral Diarrhea Virus in Necropsied Farm-Raised White-Tailed Deer (Odocoileus Virginianus) in Pennsylvania",
abstract = "Between January 1 and December 31, 2005 gross and histologic examinations were performed on carcasses of 61 farm-raised white-tailed deer originating from Pennsylvania. Single-tube real-time reverse transcription polymerase chain reaction (real-time RT-PCR) for the detection of bovine viral diarrhea virus type 1 (BVDV-1) and type 2 (BVDV-2) was performed on each animal. Virus isolation was performed on tissue samples from 25 of 61 animals. Immunohistochemical (IHC) staining of ear-notch skin to identify BVDV antigen was performed on each animal. All tissues samples tested negative for both BVDV-1 and BVDV-2 by real-time RT-PCR, virus isolation, and IHC. Gross or histopathologic lesions suggestive of BVDV infection were not detected. Results of this study suggest that BVD is not a common cause of mortality in farm-raised white-tailed deer in Pennsylvania.",
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Failure to Detect Bovine Viral Diarrhea Virus in Necropsied Farm-Raised White-Tailed Deer (Odocoileus Virginianus) in Pennsylvania. / Brooks, Jason W.; Key, Douglas W.; Hattel, Arthur L.; Hovingh, Ernest P.; Peterson, Ryan; Shaw, Daniel P.; Fisher, Jenny S.

In: Journal of Veterinary Diagnostic Investigation, Vol. 19, No. 3, 01.12.2007, p. 298-300.

Research output: Contribution to journalArticle

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