Failure to Detect Bovine Viral Diarrhea Virus in Necropsied Farm-Raised White-Tailed Deer (Odocoileus Virginianus) in Pennsylvania

Jason W. Brooks, Douglas W. Key, Arthur L. Hattel, Ernest P. Hovingh, Ryan Peterson, Daniel P. Shaw, Jenny S. Fisher

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Between January 1 and December 31, 2005 gross and histologic examinations were performed on carcasses of 61 farm-raised white-tailed deer originating from Pennsylvania. Single-tube real-time reverse transcription polymerase chain reaction (real-time RT-PCR) for the detection of bovine viral diarrhea virus type 1 (BVDV-1) and type 2 (BVDV-2) was performed on each animal. Virus isolation was performed on tissue samples from 25 of 61 animals. Immunohistochemical (IHC) staining of ear-notch skin to identify BVDV antigen was performed on each animal. All tissues samples tested negative for both BVDV-1 and BVDV-2 by real-time RT-PCR, virus isolation, and IHC. Gross or histopathologic lesions suggestive of BVDV infection were not detected. Results of this study suggest that BVD is not a common cause of mortality in farm-raised white-tailed deer in Pennsylvania.

Original languageEnglish (US)
Pages (from-to)298-300
Number of pages3
JournalJournal of Veterinary Diagnostic Investigation
Volume19
Issue number3
DOIs
StatePublished - Dec 1 2007

All Science Journal Classification (ASJC) codes

  • veterinary(all)

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