Fall armyworm-associated gut bacteria modulate plant defense responses

Flor E. Acevedo, Michelle Peiffer, Ching Wen Tan, Bruce Stanley, Anne Stanley, Jie Wang, Asher G. Jones, Kelli Hoover, Cristina Rosa, Dawn S. Luthe, Gary Felton

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid-mediated defense responses in plants. This study investigated the effects of bacteria from oral secretions of the fall armyworm Spodoptera frugiperda on herbivore-induced defenses in tomato and maize plants. Using culture-dependent methods, we identified seven different bacterial isolates belonging to the family Enterobacteriacea from the oral secretions of field-collected caterpillars. Two isolates, Pantoea ananatis and Enterobacteriaceae-1, downregulated the activity of the plant defensive proteins polyphenol oxidase and trypsin proteinase inhibitors (trypsin PI) but upregulated peroxidase (POX) activity in tomato. A Raoultella sp. and a Klebsiella sp. downregulated POX but upregulated trypsin PI in this plant species. Conversely, all of these bacterial isolates upregulated the expression of the herbivore-induced maize proteinase inhibitor (mpi) gene in maize. Plant treatment with P. ananatis and Enterobacteriaceae-1 enhanced caterpillar growth on tomato but diminished their growth on maize plants. Our results highlight the importance of herbivore-associated microbes and their ability to mediate insect plant interactions differently in host plants fed on by the same herbivore.

Original languageEnglish (US)
Pages (from-to)127-137
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume30
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Spodoptera frugiperda
digestive system
Herbivory
Bacteria
herbivores
proteinase inhibitors
Pantoea ananatis
bacteria
Zea mays
mouth
corn
Lycopersicon esculentum
secretion
tomatoes
Enterobacteriaceae
Insects
trypsin
Raoultella
insect larvae
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cite this

Acevedo, Flor E. ; Peiffer, Michelle ; Tan, Ching Wen ; Stanley, Bruce ; Stanley, Anne ; Wang, Jie ; Jones, Asher G. ; Hoover, Kelli ; Rosa, Cristina ; Luthe, Dawn S. ; Felton, Gary. / Fall armyworm-associated gut bacteria modulate plant defense responses. In: Molecular Plant-Microbe Interactions. 2017 ; Vol. 30, No. 2. pp. 127-137.
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Fall armyworm-associated gut bacteria modulate plant defense responses. / Acevedo, Flor E.; Peiffer, Michelle; Tan, Ching Wen; Stanley, Bruce; Stanley, Anne; Wang, Jie; Jones, Asher G.; Hoover, Kelli; Rosa, Cristina; Luthe, Dawn S.; Felton, Gary.

In: Molecular Plant-Microbe Interactions, Vol. 30, No. 2, 01.02.2017, p. 127-137.

Research output: Contribution to journalArticle

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