False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown

Kamyar Akbari Roshan, Zifan Tang, Weihua Guan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigate the current transport characteristics in the electrolyte-dielectric-electrolyte structure commonly used in the in-situ controlled breakdown (CBD) fabrication of solid-state nanopores. It is found that the stochastic breakdown process could lead to fidelity issues of false positives (an incorrect indication of a true nanopore formation) and false negatives (inability to detect initial nanopore formation). Robust and deterministic detection of initial physical breakdown to alleviate false positives and false negatives is critical for precise nanopore size control. To this end, we report a high fidelity moving ZScore method based CBD fabrication of solid-state nanopore. We demonstrate 100% success rate of realizing the initial nanopore conductance of 3±1 nS (corresponds to the size of 1.7±0.6 nm) regardless of the dielectric membrane characteristics. Our study also elucidates the Joule heating is the dominant mechanism for electric field-based nanopore enlargement. Single DNA molecule sensing using nanopores fabricated by this method was successfully demonstrated. We anticipate the moving ZScore based CBD method could enable broader access to the solid state nanopore-based single molecule analysis.

Original languageEnglish (US)
Title of host publication2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1756-1759
Number of pages4
ISBN (Electronic)9781728120072
DOIs
StatePublished - Jun 1 2019
Event20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII - Berlin, Germany
Duration: Jun 23 2019Jun 27 2019

Publication series

Name2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

Conference

Conference20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII
CountryGermany
CityBerlin
Period6/23/196/27/19

Fingerprint

Nanopore
Nanopores
Adaptive Learning
Electric breakdown
False Positive
learning
Breakdown
Fabrication
breakdown
fabrication
solid state
electrolytes
Electrolyte
Joule heating
Fidelity
Electrolytes
molecules
indication
deoxyribonucleic acid
Joule Heating

All Science Journal Classification (ASJC) codes

  • Process Chemistry and Technology
  • Spectroscopy
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Electronic, Optical and Magnetic Materials
  • Control and Optimization
  • Instrumentation

Cite this

Roshan, K. A., Tang, Z., & Guan, W. (2019). False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII (pp. 1756-1759). [8808631] (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2019.8808631
Roshan, Kamyar Akbari ; Tang, Zifan ; Guan, Weihua. / False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown. 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 1756-1759 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).
@inproceedings{3dbf439732ad4d72a3a7c0a7c005b9ff,
title = "False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown",
abstract = "We investigate the current transport characteristics in the electrolyte-dielectric-electrolyte structure commonly used in the in-situ controlled breakdown (CBD) fabrication of solid-state nanopores. It is found that the stochastic breakdown process could lead to fidelity issues of false positives (an incorrect indication of a true nanopore formation) and false negatives (inability to detect initial nanopore formation). Robust and deterministic detection of initial physical breakdown to alleviate false positives and false negatives is critical for precise nanopore size control. To this end, we report a high fidelity moving ZScore method based CBD fabrication of solid-state nanopore. We demonstrate 100{\%} success rate of realizing the initial nanopore conductance of 3±1 nS (corresponds to the size of 1.7±0.6 nm) regardless of the dielectric membrane characteristics. Our study also elucidates the Joule heating is the dominant mechanism for electric field-based nanopore enlargement. Single DNA molecule sensing using nanopores fabricated by this method was successfully demonstrated. We anticipate the moving ZScore based CBD method could enable broader access to the solid state nanopore-based single molecule analysis.",
author = "Roshan, {Kamyar Akbari} and Zifan Tang and Weihua Guan",
year = "2019",
month = "6",
day = "1",
doi = "10.1109/TRANSDUCERS.2019.8808631",
language = "English (US)",
series = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "1756--1759",
booktitle = "2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII",
address = "United States",

}

Roshan, KA, Tang, Z & Guan, W 2019, False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown. in 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII., 8808631, 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Institute of Electrical and Electronics Engineers Inc., pp. 1756-1759, 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII, Berlin, Germany, 6/23/19. https://doi.org/10.1109/TRANSDUCERS.2019.8808631

False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown. / Roshan, Kamyar Akbari; Tang, Zifan; Guan, Weihua.

2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc., 2019. p. 1756-1759 8808631 (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown

AU - Roshan, Kamyar Akbari

AU - Tang, Zifan

AU - Guan, Weihua

PY - 2019/6/1

Y1 - 2019/6/1

N2 - We investigate the current transport characteristics in the electrolyte-dielectric-electrolyte structure commonly used in the in-situ controlled breakdown (CBD) fabrication of solid-state nanopores. It is found that the stochastic breakdown process could lead to fidelity issues of false positives (an incorrect indication of a true nanopore formation) and false negatives (inability to detect initial nanopore formation). Robust and deterministic detection of initial physical breakdown to alleviate false positives and false negatives is critical for precise nanopore size control. To this end, we report a high fidelity moving ZScore method based CBD fabrication of solid-state nanopore. We demonstrate 100% success rate of realizing the initial nanopore conductance of 3±1 nS (corresponds to the size of 1.7±0.6 nm) regardless of the dielectric membrane characteristics. Our study also elucidates the Joule heating is the dominant mechanism for electric field-based nanopore enlargement. Single DNA molecule sensing using nanopores fabricated by this method was successfully demonstrated. We anticipate the moving ZScore based CBD method could enable broader access to the solid state nanopore-based single molecule analysis.

AB - We investigate the current transport characteristics in the electrolyte-dielectric-electrolyte structure commonly used in the in-situ controlled breakdown (CBD) fabrication of solid-state nanopores. It is found that the stochastic breakdown process could lead to fidelity issues of false positives (an incorrect indication of a true nanopore formation) and false negatives (inability to detect initial nanopore formation). Robust and deterministic detection of initial physical breakdown to alleviate false positives and false negatives is critical for precise nanopore size control. To this end, we report a high fidelity moving ZScore method based CBD fabrication of solid-state nanopore. We demonstrate 100% success rate of realizing the initial nanopore conductance of 3±1 nS (corresponds to the size of 1.7±0.6 nm) regardless of the dielectric membrane characteristics. Our study also elucidates the Joule heating is the dominant mechanism for electric field-based nanopore enlargement. Single DNA molecule sensing using nanopores fabricated by this method was successfully demonstrated. We anticipate the moving ZScore based CBD method could enable broader access to the solid state nanopore-based single molecule analysis.

UR - http://www.scopus.com/inward/record.url?scp=85071927085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071927085&partnerID=8YFLogxK

U2 - 10.1109/TRANSDUCERS.2019.8808631

DO - 10.1109/TRANSDUCERS.2019.8808631

M3 - Conference contribution

AN - SCOPUS:85071927085

T3 - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

SP - 1756

EP - 1759

BT - 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII

PB - Institute of Electrical and Electronics Engineers Inc.

ER -

Roshan KA, Tang Z, Guan W. False Negative and False Positive Free Nanopore Fabrication Via Adaptive Learning of the Controlled Dielectric Breakdown. In 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII. Institute of Electrical and Electronics Engineers Inc. 2019. p. 1756-1759. 8808631. (2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems and Eurosensors XXXIII, TRANSDUCERS 2019 and EUROSENSORS XXXIII). https://doi.org/10.1109/TRANSDUCERS.2019.8808631