Feasibility of percutaneous gene transfer to an atrophic nonunion in a rabbit

Christian Lattermann, Axel W. Baltzer, Boris A. Zelle, Janey D. Whalen, Christopher Niyibizi, Paul D. Robbins, Christopher H. Evans, Gary S. Gruen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Treatment of atrophic nonunions is a challenge to orthopaedic surgeons. Growth factors potentially are valuable factors for improvement of tissue healing. The use of growth factors, however, is limited by their short half-lives. Gene therapy lias the potential to improve the treatment. This study aimed to establish and validate an atrophic nonunion model in a rabbit for the use of a percutaneous in vivo gene therapy protocol. An atrophic tibial nonunion was established in 24 New Zealand White rabbits. Radiologic and histologic followup was for 64 weeks. The rabbit tibias showed no radiologic or histologic signs of healing. In addition, an adenoviral vector carrying a marker gene was injected percutaneously into the nonunion site in 12 rabbits. Expression of the marker gene was assessed for as many as 4 weeks. The percutaneous gene delivery resulted in transgene expression in the nonunion site for as many as 4 weeks. The described model reliably leads to an atrophic tibial nonunion in rabbits. Adenoviral percutaneous gene delivery into the nonunion site is feasible and leads to transgene expression locally for at least 1 month. This study provides investigators with a reliable and reproducible model of an atrophic nonunion.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalClinical Orthopaedics and Related Research
Issue number425
DOIs
StatePublished - Jan 1 2004

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Rabbits
Genes
Transgenes
Genetic Therapy
Intercellular Signaling Peptides and Proteins
Thromboplastin
Tibia
Research Personnel
Gene Expression
Therapeutics

All Science Journal Classification (ASJC) codes

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Lattermann, C., Baltzer, A. W., Zelle, B. A., Whalen, J. D., Niyibizi, C., Robbins, P. D., ... Gruen, G. S. (2004). Feasibility of percutaneous gene transfer to an atrophic nonunion in a rabbit. Clinical Orthopaedics and Related Research, (425), 237-243. https://doi.org/10.1097/00003086-200408000-00034
Lattermann, Christian ; Baltzer, Axel W. ; Zelle, Boris A. ; Whalen, Janey D. ; Niyibizi, Christopher ; Robbins, Paul D. ; Evans, Christopher H. ; Gruen, Gary S. / Feasibility of percutaneous gene transfer to an atrophic nonunion in a rabbit. In: Clinical Orthopaedics and Related Research. 2004 ; No. 425. pp. 237-243.
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Lattermann, C, Baltzer, AW, Zelle, BA, Whalen, JD, Niyibizi, C, Robbins, PD, Evans, CH & Gruen, GS 2004, 'Feasibility of percutaneous gene transfer to an atrophic nonunion in a rabbit', Clinical Orthopaedics and Related Research, no. 425, pp. 237-243. https://doi.org/10.1097/00003086-200408000-00034

Feasibility of percutaneous gene transfer to an atrophic nonunion in a rabbit. / Lattermann, Christian; Baltzer, Axel W.; Zelle, Boris A.; Whalen, Janey D.; Niyibizi, Christopher; Robbins, Paul D.; Evans, Christopher H.; Gruen, Gary S.

In: Clinical Orthopaedics and Related Research, No. 425, 01.01.2004, p. 237-243.

Research output: Contribution to journalArticle

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AU - Gruen, Gary S.

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