Fed-batch fermentation for human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors

Duygu Ercan, Ali Demirci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lysozyme is a lytic enzyme, which has antimicrobial, antiviral, anti-inflammatory activities. Enhancement of lysozyme production is required to meet its current demand in pharmaceutical and food industry. Egg white lysozyme is the major commercial source of lysozyme. However, egg-white lysozyme poses immunological problems when applied to human beings and it has a four-times less specific activity than human lysozyme. There are are several approaches for human lysozyme production and microbial fermentation is one of them. Biofilm reactors with plastic composite support have been evaluated for human lysozyme production by fermentation of Kluyveromyces lactis K7 in our earlier study, which demonstrated significant improvement in batch fermentation of human lysozyme. Biofilm reactor with plastic composite support is a passive immobilization reactor, which includes attachment of cells onto support without using any chemical agent. Fed-batch mode includes a step to feed the reactor with additional carbon source during the fermentation process. Extra substrate availability in fed-batch bioreactors could provide higher production levels and yields and prevent substrate inhibition. Therefore, this study was undertaken to evaluate different carbon source addition times and different initial carbon sources and their concentrations to increase the human lysozyme production by K. lactis K7 in biofilm reactor in fed-batch fermentation. Result showed that addition of carbon source in the log phase (177 U/ml) than the late log phase (174 U/ml) provided higher lysozyme production. Moreover, human lysozyme production by K. lactis K7 with glucose as initial carbon source (187 U/ml) was found higher than the production just with lactose (178 U/ml).

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2013, ASABE 2013
PublisherAmerican Society of Agricultural and Biological Engineers
Pages1674-1680
Number of pages7
ISBN (Print)9781627486651
StatePublished - Jan 1 2013
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2013 - Kansas City, MO, United States
Duration: Jul 21 2013Jul 24 2013

Publication series

NameAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2013, ASABE 2013
Volume2

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2013
CountryUnited States
CityKansas City, MO
Period7/21/137/24/13

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All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Bioengineering

Cite this

Ercan, D., & Demirci, A. (2013). Fed-batch fermentation for human lysozyme production by Kluyveromyces lactis K7 in biofilm reactors. In American Society of Agricultural and Biological Engineers Annual International Meeting 2013, ASABE 2013 (pp. 1674-1680). (American Society of Agricultural and Biological Engineers Annual International Meeting 2013, ASABE 2013; Vol. 2). American Society of Agricultural and Biological Engineers.