Feedback Inhibition of Tyrosine Hydroxylase from Five Regions of Rat Brain by 2‐Hydroxyestradiol and Dihy droxy pheny lalanine

Thomas Lloyd, Barbara Jones Ebersole

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Abstract: Inhibition of tyrosine hydroxylase from five regions of rat brain by a model catecholamine, DOPA and a model catecholestrogen, 2‐hydroxyestradiol, was examined. Tyrosine hydroxylase preparations from amygdala, preoptic, hypothalamic, striatal and hippocampal regions were freed of small molecules by gel filtration before use. The feedback inhibition of tyrosine hydroxylase by the two model catechols was studied using a spectrum of reduced pterin cofactors, including tetrahydrobiopterin, 6‐methyl‐tetrahydropterin and 6, 7‐dimethyltetrahydropterin. Micromolar levels of either inhibitor produced marked inhibition of tyrosine hydroxylase from all regions when subsaturating levels of the endogenous cofactor, tetrahydrobiopterin, were used.

Original languageEnglish (US)
Pages (from-to)726-731
Number of pages6
JournalJournal of neurochemistry
Volume34
Issue number3
DOIs
StatePublished - Jan 1 1980

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Tyrosine 3-Monooxygenase
Rats
Brain
Feedback
Catechol Estrogens
Catechols
Pterins
Corpus Striatum
Amygdala
Catecholamines
Gel Chromatography
Gels
Molecules
sapropterin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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Feedback Inhibition of Tyrosine Hydroxylase from Five Regions of Rat Brain by 2‐Hydroxyestradiol and Dihy droxy pheny lalanine. / Lloyd, Thomas; Ebersole, Barbara Jones.

In: Journal of neurochemistry, Vol. 34, No. 3, 01.01.1980, p. 726-731.

Research output: Contribution to journalArticle

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