Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel α(1c) subunit gene

Wansheng Liu, Nikolai M. Soldatov, Ingemar Gustavsson, Bhanu P. Chowdhary

Research output: Contribution to journalArticle

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Abstract

Human L-type Ca2+ channel α(1c) subunit gene (CACNL1A1) maps to the distal region of chromosome 12p13, and is composed of ≃ 50 exons spanning over 150 kb of the human genome as estimated by restriction map analysis. However, the structure and the total length of the 3'-end of the gene is not clear because the size of several big introns remains unknown. Here the fiber-FISH technique was used to determine the relative order and size of eight partial genomic DNA clones from the central and 3'-terminal regions of CACNL1A1. The total physical distance of this region, including the size and gap distances between the clones were re-estimated. The results show that the physical order of the tested clones was 5'- g14-5 > g12-2 > g10-8 > g4-5 > g16-7 > g8-3 > g12-5 > g6-20-3'. Their individual sizes vary between 6.7 and 21.9 kb. Clones g6-20 and g12-5, both containing repetitive exon 45/46-like element, were found to be located within 59.1 kb downstream of g8-3 containing earlier identified polyadenylation site, i.e. 229.5 kb away from clone g14-5 (exons 10, 11). The possible implications of this structural complexity is discussed.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalHereditas
Volume129
Issue number2
StatePublished - 1998

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Clone Cells
Exons
Genes
Polyadenylation
Human Genome
Fluorescence In Situ Hybridization
Introns
Chromosomes
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Liu, W., Soldatov, N. M., Gustavsson, I., & Chowdhary, B. P. (1998). Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel α(1c) subunit gene. Hereditas, 129(2), 169-175.
Liu, Wansheng ; Soldatov, Nikolai M. ; Gustavsson, Ingemar ; Chowdhary, Bhanu P. / Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel α(1c) subunit gene. In: Hereditas. 1998 ; Vol. 129, No. 2. pp. 169-175.
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abstract = "Human L-type Ca2+ channel α(1c) subunit gene (CACNL1A1) maps to the distal region of chromosome 12p13, and is composed of ≃ 50 exons spanning over 150 kb of the human genome as estimated by restriction map analysis. However, the structure and the total length of the 3'-end of the gene is not clear because the size of several big introns remains unknown. Here the fiber-FISH technique was used to determine the relative order and size of eight partial genomic DNA clones from the central and 3'-terminal regions of CACNL1A1. The total physical distance of this region, including the size and gap distances between the clones were re-estimated. The results show that the physical order of the tested clones was 5'- g14-5 > g12-2 > g10-8 > g4-5 > g16-7 > g8-3 > g12-5 > g6-20-3'. Their individual sizes vary between 6.7 and 21.9 kb. Clones g6-20 and g12-5, both containing repetitive exon 45/46-like element, were found to be located within 59.1 kb downstream of g8-3 containing earlier identified polyadenylation site, i.e. 229.5 kb away from clone g14-5 (exons 10, 11). The possible implications of this structural complexity is discussed.",
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Liu, W, Soldatov, NM, Gustavsson, I & Chowdhary, BP 1998, 'Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel α(1c) subunit gene', Hereditas, vol. 129, no. 2, pp. 169-175.

Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel α(1c) subunit gene. / Liu, Wansheng; Soldatov, Nikolai M.; Gustavsson, Ingemar; Chowdhary, Bhanu P.

In: Hereditas, Vol. 129, No. 2, 1998, p. 169-175.

Research output: Contribution to journalArticle

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T1 - Fiber-FISH analysis of the 3'-terminal region of the human L-type Ca2+ channel α(1c) subunit gene

AU - Liu, Wansheng

AU - Soldatov, Nikolai M.

AU - Gustavsson, Ingemar

AU - Chowdhary, Bhanu P.

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