Fidelity of in Vitro DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase

James A. Peliska, Stephen J. Benkovic

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The fidelity of DNA strand transfer reactions catalyzed by human immunodeficiency virus type 1 reverse transcriptase has been studied in vitro. A model system involving two sequential DNA strand transfers was developed to simulate the process of forced copy-choice recombination. A propensity for nucleotide misincorporation at the junction of the strand transfer, as determined by DNA sequencing of the reaction products, was found consistent with a model involving the addition of nontemplate-directed nucleotides prior to the transfer of nascent DNA onto the accepting RNA template [Peliska, J. A., & Benkovic, S. J. (1992) Science 258, 1112]. The kinetic and mechanistic factors that may dictate which nucleotide bases are incorporated at recombination sites during strand transfer and the possible consequences of recombination-induced mutagenesis in vivo are discussed.

Original languageEnglish (US)
Pages (from-to)3890-3895
Number of pages6
JournalBiochemistry
Volume33
Issue number13
DOIs
StatePublished - Apr 1 1994

All Science Journal Classification (ASJC) codes

  • Biochemistry

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