Fidelity of in Vitro DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase

James A. Peliska, Stephen Benkovic

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The fidelity of DNA strand transfer reactions catalyzed by human immunodeficiency virus type 1 reverse transcriptase has been studied in vitro. A model system involving two sequential DNA strand transfers was developed to simulate the process of forced copy-choice recombination. A propensity for nucleotide misincorporation at the junction of the strand transfer, as determined by DNA sequencing of the reaction products, was found consistent with a model involving the addition of nontemplate-directed nucleotides prior to the transfer of nascent DNA onto the accepting RNA template [Peliska, J. A., & Benkovic, S. J. (1992) Science 258, 1112]. The kinetic and mechanistic factors that may dictate which nucleotide bases are incorporated at recombination sites during strand transfer and the possible consequences of recombination-induced mutagenesis in vivo are discussed.

Original languageEnglish (US)
Pages (from-to)3890-3895
Number of pages6
JournalBiochemistry
Volume33
Issue number13
DOIs
StatePublished - Apr 1 1994

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Genetic Recombination
Nucleotides
DNA
DNA Sequence Analysis
Mutagenesis
HIV-1
Reaction products
RNA
Kinetics
In Vitro Techniques
Human immunodeficiency virus 1 reverse transcriptase

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Peliska, James A. ; Benkovic, Stephen. / Fidelity of in Vitro DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase. In: Biochemistry. 1994 ; Vol. 33, No. 13. pp. 3890-3895.
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Fidelity of in Vitro DNA Strand Transfer Reactions Catalyzed by HIV-1 Reverse Transcriptase. / Peliska, James A.; Benkovic, Stephen.

In: Biochemistry, Vol. 33, No. 13, 01.04.1994, p. 3890-3895.

Research output: Contribution to journalArticle

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