Field survey of Cucumber mosaic virus subgroups I and II in crop plants in Costa Rica

M. J. Hord, A. García, H. Villalobos, C. Rivera, G. Macaya, Marilyn J. Roossinck

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Leaf samples were collected from cucurbit and solanaceous crop plants and Musa spp. in 28 locations in five provinces of Costa Rica during the period from January to October 1996. Sampling sites were selected in dry, humid, and moist tropical regions ranging in altitude from 50 to 2,100 m above sea level. RNA-enriched total nucleic acid solutions were spotted onto nylon membranes and hybridized to RNA probes specific for Cucumber mosaic virus (CMV) subgroups I or II. The presence of CMV was confirmed in 13 crops in 23 of the 28 sampling sites. CMV subgroup I was found to predominate in Costa Rica. CMV subgroup II was detected in the Atlantic region only, and in only 1 out of 113 CMV-positive samples.

Original languageEnglish (US)
Pages (from-to)952-954
Number of pages3
JournalPlant disease
Volume85
Issue number9
DOIs
StatePublished - Jan 1 2001

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Cucumber mosaic virus
Costa Rica
crops
RNA probes
sampling
Musa
Cucurbitaceae
nylon
nucleic acids
sea level
tropics
RNA
leaves

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Hord, M. J. ; García, A. ; Villalobos, H. ; Rivera, C. ; Macaya, G. ; Roossinck, Marilyn J. / Field survey of Cucumber mosaic virus subgroups I and II in crop plants in Costa Rica. In: Plant disease. 2001 ; Vol. 85, No. 9. pp. 952-954.
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Field survey of Cucumber mosaic virus subgroups I and II in crop plants in Costa Rica. / Hord, M. J.; García, A.; Villalobos, H.; Rivera, C.; Macaya, G.; Roossinck, Marilyn J.

In: Plant disease, Vol. 85, No. 9, 01.01.2001, p. 952-954.

Research output: Contribution to journalArticle

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AU - Roossinck, Marilyn J.

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AB - Leaf samples were collected from cucurbit and solanaceous crop plants and Musa spp. in 28 locations in five provinces of Costa Rica during the period from January to October 1996. Sampling sites were selected in dry, humid, and moist tropical regions ranging in altitude from 50 to 2,100 m above sea level. RNA-enriched total nucleic acid solutions were spotted onto nylon membranes and hybridized to RNA probes specific for Cucumber mosaic virus (CMV) subgroups I or II. The presence of CMV was confirmed in 13 crops in 23 of the 28 sampling sites. CMV subgroup I was found to predominate in Costa Rica. CMV subgroup II was detected in the Atlantic region only, and in only 1 out of 113 CMV-positive samples.

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