TY - JOUR
T1 - Finding order in a bustling construction zone
T2 - quantitative imaging and analysis of cell wall assembly in plants
AU - Anderson, Charles T.
N1 - Funding Information:
Thanks to members of the Anderson Lab and the Center for Lignocellulose Structure and Formation for helpful discussions, and to the reviewers and editor, as well as William Barnes, for helpful suggestions on the manuscript. This work was supported as part of the Center for Lignocellulose Structure and Formation , an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences under Award # DE-SC0001090.
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2018/12
Y1 - 2018/12
N2 - Assembly of polysaccharide-based walls by plant cells involves the rapid synthesis, trafficking, and deposition of complex biopolymers, but how these events are controlled and coordinated to achieve a strong, resilient extracellular matrix has remained obscure for decades. Recent quantitative analyses of fluorescence microscopy data have revealed details of the trafficking and synthetic activity of cellulose synthases, and new methods for labeling matrix polymers have unveiled aspects of their regulated deposition in the wall. Detailed studies of the identity, architecture, activity, and trafficking of the proteins and protein complexes that synthesize wall polymers, combined with advances in image acquisition and analysis, will aid future efforts to dissect wall assembly.
AB - Assembly of polysaccharide-based walls by plant cells involves the rapid synthesis, trafficking, and deposition of complex biopolymers, but how these events are controlled and coordinated to achieve a strong, resilient extracellular matrix has remained obscure for decades. Recent quantitative analyses of fluorescence microscopy data have revealed details of the trafficking and synthetic activity of cellulose synthases, and new methods for labeling matrix polymers have unveiled aspects of their regulated deposition in the wall. Detailed studies of the identity, architecture, activity, and trafficking of the proteins and protein complexes that synthesize wall polymers, combined with advances in image acquisition and analysis, will aid future efforts to dissect wall assembly.
UR - http://www.scopus.com/inward/record.url?scp=85051244771&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051244771&partnerID=8YFLogxK
U2 - 10.1016/j.pbi.2018.07.014
DO - 10.1016/j.pbi.2018.07.014
M3 - Review article
C2 - 30107305
AN - SCOPUS:85051244771
VL - 46
SP - 62
EP - 67
JO - Current Opinion in Plant Biology
JF - Current Opinion in Plant Biology
SN - 1369-5266
ER -