Fine specificity and antigen-receptor expression among influenza virus-specific cytolytic T lymphocyte clones

T. J. Braciale, T. J. Henkel, Aron Lukacher, V. L. Braciale

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Influenza virus stimulates a vigorous cytolytic T lymphocyte (CTL) response in the mouse that is directed to several virion polypeptides. This report examines the fine specificity of a panel of murine influenza-specific CTL clones restricted by MHC class I products of the H-2(d) haplotype. Ten of 22 A/JAPAN/305/57-specific CTL clones analyzed were directed to the A/JAPAN/305/57 hemagglutinin protein as detected by using target cells infected with a recombinant vaccinia virus containing hemagglutinin gene. Based on their fine specificity of hemagglutinin recognition, these clones defined four functional epitopes on the hemagglutinin. The remaining 12 cytolytic clones exhibited cross-reactivity for type A influenza viruses of the major human subtypes, and approximately 60% of these clones were directed to the nucleocapsid protein. KJ16-133 monoclonal antibody analysis of the utilization of the T cell receptor V(β)8 gene segment subfamily revealed that members of this V(β) gene subfamily are expressed by both hemagglutinin- and nucleocapsid-specific MHC class I-restricted CTL (and by influenza-specific MHC class II-restricted T lymphocytes as well). These results suggest that CTL detect several distinct antigenic sites on the hemagglutinin. In addition, these results reveal no direct correlation between viral antigenic specificity and V(β) gene expression by these virus-specific CTL clones.

Original languageEnglish (US)
Pages (from-to)995-1002
Number of pages8
JournalJournal of Immunology
Volume137
Issue number3
StatePublished - Jan 1 1986

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Antigen Receptors
Orthomyxoviridae
Hemagglutinins
Clone Cells
T-Lymphocytes
Human Influenza
Epitopes
Genes
Nucleocapsid Proteins
Nucleocapsid
Vaccinia virus
Influenza A virus
T-Cell Antigen Receptor
Virion
Haplotypes
Monoclonal Antibodies
Viruses
Gene Expression
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

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title = "Fine specificity and antigen-receptor expression among influenza virus-specific cytolytic T lymphocyte clones",
abstract = "Influenza virus stimulates a vigorous cytolytic T lymphocyte (CTL) response in the mouse that is directed to several virion polypeptides. This report examines the fine specificity of a panel of murine influenza-specific CTL clones restricted by MHC class I products of the H-2(d) haplotype. Ten of 22 A/JAPAN/305/57-specific CTL clones analyzed were directed to the A/JAPAN/305/57 hemagglutinin protein as detected by using target cells infected with a recombinant vaccinia virus containing hemagglutinin gene. Based on their fine specificity of hemagglutinin recognition, these clones defined four functional epitopes on the hemagglutinin. The remaining 12 cytolytic clones exhibited cross-reactivity for type A influenza viruses of the major human subtypes, and approximately 60{\%} of these clones were directed to the nucleocapsid protein. KJ16-133 monoclonal antibody analysis of the utilization of the T cell receptor V(β)8 gene segment subfamily revealed that members of this V(β) gene subfamily are expressed by both hemagglutinin- and nucleocapsid-specific MHC class I-restricted CTL (and by influenza-specific MHC class II-restricted T lymphocytes as well). These results suggest that CTL detect several distinct antigenic sites on the hemagglutinin. In addition, these results reveal no direct correlation between viral antigenic specificity and V(β) gene expression by these virus-specific CTL clones.",
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Fine specificity and antigen-receptor expression among influenza virus-specific cytolytic T lymphocyte clones. / Braciale, T. J.; Henkel, T. J.; Lukacher, Aron; Braciale, V. L.

In: Journal of Immunology, Vol. 137, No. 3, 01.01.1986, p. 995-1002.

Research output: Contribution to journalArticle

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AB - Influenza virus stimulates a vigorous cytolytic T lymphocyte (CTL) response in the mouse that is directed to several virion polypeptides. This report examines the fine specificity of a panel of murine influenza-specific CTL clones restricted by MHC class I products of the H-2(d) haplotype. Ten of 22 A/JAPAN/305/57-specific CTL clones analyzed were directed to the A/JAPAN/305/57 hemagglutinin protein as detected by using target cells infected with a recombinant vaccinia virus containing hemagglutinin gene. Based on their fine specificity of hemagglutinin recognition, these clones defined four functional epitopes on the hemagglutinin. The remaining 12 cytolytic clones exhibited cross-reactivity for type A influenza viruses of the major human subtypes, and approximately 60% of these clones were directed to the nucleocapsid protein. KJ16-133 monoclonal antibody analysis of the utilization of the T cell receptor V(β)8 gene segment subfamily revealed that members of this V(β) gene subfamily are expressed by both hemagglutinin- and nucleocapsid-specific MHC class I-restricted CTL (and by influenza-specific MHC class II-restricted T lymphocytes as well). These results suggest that CTL detect several distinct antigenic sites on the hemagglutinin. In addition, these results reveal no direct correlation between viral antigenic specificity and V(β) gene expression by these virus-specific CTL clones.

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