Fish oil alters tamoxifen-modulated expression of mRNAs that encode genes related to differentiation, proliferation, metastasis, and immune response in rat mammary tumors

Lucas Tadeu Bidinotto, Ricardo López De Cicco, Johana Erika Vanegas, Julia Santucci-Pereira, John Patrick Vanden Heuvel, Sharlene Washington, Cesar Aliaga, Haifang Xu, Irma H. Russo, Andrea Manni, Karam El-Bayoumy, Jose Russo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have previously shown that a fish oil (FO)-rich diet increased the chemopreventive efficacy of tamoxifen (Tam) against N-methyl-N-nitrosourea (MNU)-induced rat mammary carcinogenesis. Herein, we provide evidence that Tam treatment modifies gene expression of mammary tumors depending upon the type of dietary fat fed to the animals. Rats initiated with MNU and treated with Tam were fed a diet rich in corn oil or FO. After 8 wk, cribriform tumors were collected and gene expression analysis was performed. Increased RNA expression of genes such as SerpinB10, Wisp2, and Apod in tumors from FO-treated rats is indicative of highly differentiated tumors. Decreased expression of H19 and Igf2 mRNA in Tam-treated groups, and Gamma Synuclein mRNA in the FO + Tam group may be related to tumor growth impairment and lower metastatic capacity. Change in the expression of genes associated with immunity in animals in the FO + Tam group may suggest a shift in the immune response. These data show that, although Tam modulates the expression of genes leading to tumor growth impairment, further modulations of genes are influenced by FO. FO modulation of Tam changes in gene expression accounts for its enhancing chemopreventive effect against MNU-induced mammary carcinogenesis. Supplemental materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer to view the supplemental file.

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalNutrition and cancer
Volume64
Issue number7
DOIs
StatePublished - Oct 1 2012

Fingerprint

Fish Oils
Tamoxifen
Breast Neoplasms
Neoplasm Metastasis
Messenger RNA
Gene Expression
Genes
Neoplasms
gamma-Synuclein
Carcinogenesis
Breast
Diet
Methylnitrosourea
Corn Oil
Dietary Fats
Growth
Immunity
RNA

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Bidinotto, Lucas Tadeu ; De Cicco, Ricardo López ; Vanegas, Johana Erika ; Santucci-Pereira, Julia ; Vanden Heuvel, John Patrick ; Washington, Sharlene ; Aliaga, Cesar ; Xu, Haifang ; Russo, Irma H. ; Manni, Andrea ; El-Bayoumy, Karam ; Russo, Jose. / Fish oil alters tamoxifen-modulated expression of mRNAs that encode genes related to differentiation, proliferation, metastasis, and immune response in rat mammary tumors. In: Nutrition and cancer. 2012 ; Vol. 64, No. 7. pp. 991-999.
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Fish oil alters tamoxifen-modulated expression of mRNAs that encode genes related to differentiation, proliferation, metastasis, and immune response in rat mammary tumors. / Bidinotto, Lucas Tadeu; De Cicco, Ricardo López; Vanegas, Johana Erika; Santucci-Pereira, Julia; Vanden Heuvel, John Patrick; Washington, Sharlene; Aliaga, Cesar; Xu, Haifang; Russo, Irma H.; Manni, Andrea; El-Bayoumy, Karam; Russo, Jose.

In: Nutrition and cancer, Vol. 64, No. 7, 01.10.2012, p. 991-999.

Research output: Contribution to journalArticle

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AU - Bidinotto, Lucas Tadeu

AU - De Cicco, Ricardo López

AU - Vanegas, Johana Erika

AU - Santucci-Pereira, Julia

AU - Vanden Heuvel, John Patrick

AU - Washington, Sharlene

AU - Aliaga, Cesar

AU - Xu, Haifang

AU - Russo, Irma H.

AU - Manni, Andrea

AU - El-Bayoumy, Karam

AU - Russo, Jose

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