TY - JOUR
T1 - Fish Oil Enhances T Cell Function and Tumor Infiltration and Is Correlated with a Cancer Prevention Effect in HER-2/neu but Not PyMT Transgenic Mice
AU - Turbitt, William J.
AU - Black, Adam J.
AU - Collins, Shawntawnee D.
AU - Meng, Huicui
AU - Xu, Haifang
AU - Washington, Sharlene
AU - Aliaga, Cesar
AU - El-Bayoumy, Karam
AU - Manni, Andrea
AU - Rogers, Connie J.
N1 - Funding Information:
The authors are thankful to Samina Alam for assistance in the transport of samples. William J Turbitt and Adam J. Black are supported by the Intercollege Graduate Degree Program in Physiology at Pennsylvania State University. Shawntawnee D. Collins is supported by the Sloane Foundation and the Clinical Translational Research Institute. This work is supported in part by the Susan G. Komen Foundation, Komen Grant KG0681632.
Publisher Copyright:
© 2015, Taylor & Francis Group, LLC.
PY - 2015/8/18
Y1 - 2015/8/18
N2 - Few studies have explored the effects of omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementation on immune modulation in murine models of mammary carcinogenesis. HER-2/neu and PyMT mice were randomized to 2 dietary interventions: AIN-93G-based diet with 1) 11% of diet (per gram weight) as corn oil (CO) or 2) 10% of diet as menhaden fish oil plus 1% of diet as corn oil (FO). FO significantly reduced the incidence and multiplicity of tumors (P < 0.001) in HER-2/neu, but not PyMT mice. FO-fed mice had significantly larger splenocyte counts than CO-fed mice in both the HER-2/neu and PyMT models; and in both models this was comprised of an increase in most cell types, including Gr-1+/CD11b+ cells. T cells from FO-fed HER-2/neu mice produced significantly more interleukin-2 (P = 0.004) and interferon-γ (P = 0.012) in response to in vitro stimulation with anti-CD3 (0.5 g/ml). Lastly, FO-fed HER-2/neu mice had significantly more tumor immune infiltrates than CO-fed mice, including NK1.1+, F4/80+, and Gr-1+/CD11b+ cells (P ≤ 0.05). Greater Th1 cytokine production and significantly more tumor immune infiltrates in FO-fed Her2/neu mice may account for the cancer prevention effect of fish oil in this model.
AB - Few studies have explored the effects of omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementation on immune modulation in murine models of mammary carcinogenesis. HER-2/neu and PyMT mice were randomized to 2 dietary interventions: AIN-93G-based diet with 1) 11% of diet (per gram weight) as corn oil (CO) or 2) 10% of diet as menhaden fish oil plus 1% of diet as corn oil (FO). FO significantly reduced the incidence and multiplicity of tumors (P < 0.001) in HER-2/neu, but not PyMT mice. FO-fed mice had significantly larger splenocyte counts than CO-fed mice in both the HER-2/neu and PyMT models; and in both models this was comprised of an increase in most cell types, including Gr-1+/CD11b+ cells. T cells from FO-fed HER-2/neu mice produced significantly more interleukin-2 (P = 0.004) and interferon-γ (P = 0.012) in response to in vitro stimulation with anti-CD3 (0.5 g/ml). Lastly, FO-fed HER-2/neu mice had significantly more tumor immune infiltrates than CO-fed mice, including NK1.1+, F4/80+, and Gr-1+/CD11b+ cells (P ≤ 0.05). Greater Th1 cytokine production and significantly more tumor immune infiltrates in FO-fed Her2/neu mice may account for the cancer prevention effect of fish oil in this model.
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U2 - 10.1080/01635581.2015.1060351
DO - 10.1080/01635581.2015.1060351
M3 - Article
C2 - 26226376
AN - SCOPUS:84939653892
VL - 67
SP - 965
EP - 975
JO - Nutrition and Cancer
JF - Nutrition and Cancer
SN - 0163-5581
IS - 6
ER -