Fitness and competitive ability of an azoxystrobin-resistant G143A mutant of magnaporthe oryzae from perennial ryegrass

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ma, B., and Uddin, W. 2009. Fitness and competitive ability of an azoxystrobin-resistant G143A mutant of Magnaporthe oryzae from perennial ryegrass. Plant Dis. 93:1044-1049. Development of azoxystrobin resistance in Magnaporthe oryzae from perennial ryegrass has been reported in certain locations in the United States, and possible development of resistance in additional areas is a major concern in the golf course industry. The study was undertaken to evaluate the relative fitness and competitive ability of a field-collected azoxystrobin-resistant G143A mutant by comparing it with a wild-type strain using detached perennial ryegrass blades. A fitness comparison experiment indicated that the disease severity of the wild-type strain was significantly higher than that of the mutant; however, the mutant produced greater secondary inoculum. When inoculated with three mixed populations of resistant and wild-type strains at different ratios, the production of conidia by the wild-type strain increased and that of the mutant decreased after infection occurred in all three populations tested. In an experiment on the effect of various fungicides on the population initially containing 5% of the mutant, preventive application of azoxystrobin allowed 5% of the mutant to dominate the population after the infection. However, other non-quinone outside inhibitor fungicides and mixtures of azoxystrobin with contact fungicides eliminated the entire mutant. This study demonstrates that the wild-type strain of M. oryzae has a competitive advantage over the mutant within the environment tested. Mixtures and alternations of fungicides with different modes of actions may prevent rapid build-up of resistance in the gray leaf spot pathosystem.

Original languageEnglish (US)
Pages (from-to)1044-1049
Number of pages6
JournalPlant disease
Volume93
Issue number10
DOIs
StatePublished - Oct 1 2009

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Magnaporthe oryzae
Lolium perenne
mutants
fungicides
leaf spot
azoxystrobin
golf courses
infection
disease severity
conidia
mechanism of action
inoculum
industry

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

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title = "Fitness and competitive ability of an azoxystrobin-resistant G143A mutant of magnaporthe oryzae from perennial ryegrass",
abstract = "Ma, B., and Uddin, W. 2009. Fitness and competitive ability of an azoxystrobin-resistant G143A mutant of Magnaporthe oryzae from perennial ryegrass. Plant Dis. 93:1044-1049. Development of azoxystrobin resistance in Magnaporthe oryzae from perennial ryegrass has been reported in certain locations in the United States, and possible development of resistance in additional areas is a major concern in the golf course industry. The study was undertaken to evaluate the relative fitness and competitive ability of a field-collected azoxystrobin-resistant G143A mutant by comparing it with a wild-type strain using detached perennial ryegrass blades. A fitness comparison experiment indicated that the disease severity of the wild-type strain was significantly higher than that of the mutant; however, the mutant produced greater secondary inoculum. When inoculated with three mixed populations of resistant and wild-type strains at different ratios, the production of conidia by the wild-type strain increased and that of the mutant decreased after infection occurred in all three populations tested. In an experiment on the effect of various fungicides on the population initially containing 5{\%} of the mutant, preventive application of azoxystrobin allowed 5{\%} of the mutant to dominate the population after the infection. However, other non-quinone outside inhibitor fungicides and mixtures of azoxystrobin with contact fungicides eliminated the entire mutant. This study demonstrates that the wild-type strain of M. oryzae has a competitive advantage over the mutant within the environment tested. Mixtures and alternations of fungicides with different modes of actions may prevent rapid build-up of resistance in the gray leaf spot pathosystem.",
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Fitness and competitive ability of an azoxystrobin-resistant G143A mutant of magnaporthe oryzae from perennial ryegrass. / Ma, B.; Uddin, Wakar.

In: Plant disease, Vol. 93, No. 10, 01.10.2009, p. 1044-1049.

Research output: Contribution to journalArticle

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