Flaxseed-derived enterolactone is inversely associated with tumor cell proliferation in men with localized prostate cancer

Maria Azrad, Robin T. Vollmer, John Madden, Mark Dewhirst, Thomas J. Polascik, Denise C. Snyder, Mack T. Ruffin, Judd W. Moul, Dean E. Brenner, Wendy Demark-Wahnefried

Research output: Contribution to journalArticle

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Abstract

Enterolactone and enterodiol, mammalian lignans derived from dietary sources such as flaxseed, sesame seeds, kale, broccoli, and apricots, may impede tumor proliferation by inhibiting activation of nuclear factor kappa B (NFκB) and vascular endothelial growth factor (VEGF). We examined the associations between urinary enterolactone and enterodiol with prostatic tumor expression of NFκB, VEGF, and Ki67 among 147 patients with prostate cancer who participated in a presurgical trial of flaxseed supplementation (30 g/day) for ∼30 days. Urinary enterolignans and tissue biomarkers were determined by high-performance liquid chromatography and immunohistochemistry, respectively. After supplementation, we observed significant correlations between intakes of plant lignan and urinary concentrations of total enterolignans (ρ=0.677, P<.0001), enterolactone (ρ=0.676, P<.0001), and enterodiol (ρ=0.628, P<.0001). Importantly, we observed that total urinary enterolignans and enterolactone were significantly and inversely correlated with Ki67 in the tumor tissue (ρ=-0.217, P=.011, and ρ=-0.230, P=.007, respectively), and a near-significant inverse association was observed for enterodiol (ρ=-0.159, P=.064). An inverse association was observed between enterolactone and VEGF (ρ=-0.143, P=.141), although this did not reach statistical significance. We did not observe an association between enterolignans and NFκB. In conclusion, flaxseed-derived enterolignans may hinder cancer cell proliferation via VEGF-associated pathways.

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalJournal of Medicinal Food
Volume16
Issue number4
DOIs
StatePublished - Apr 1 2013

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Flax
Prostatic Neoplasms
Vascular Endothelial Growth Factor A
Cell Proliferation
NF-kappa B
Lignans
Brassica
Neoplasms
Sesamum
Seeds
Biomarkers
Immunohistochemistry
High Pressure Liquid Chromatography
2,3-bis(3'-hydroxybenzyl)butyrolactone
2,3-bis(3'-hydroxybenzyl)butane-1,4-diol

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Azrad, M., Vollmer, R. T., Madden, J., Dewhirst, M., Polascik, T. J., Snyder, D. C., ... Demark-Wahnefried, W. (2013). Flaxseed-derived enterolactone is inversely associated with tumor cell proliferation in men with localized prostate cancer. Journal of Medicinal Food, 16(4), 357-360. https://doi.org/10.1089/jmf.2012.0159
Azrad, Maria ; Vollmer, Robin T. ; Madden, John ; Dewhirst, Mark ; Polascik, Thomas J. ; Snyder, Denise C. ; Ruffin, Mack T. ; Moul, Judd W. ; Brenner, Dean E. ; Demark-Wahnefried, Wendy. / Flaxseed-derived enterolactone is inversely associated with tumor cell proliferation in men with localized prostate cancer. In: Journal of Medicinal Food. 2013 ; Vol. 16, No. 4. pp. 357-360.
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abstract = "Enterolactone and enterodiol, mammalian lignans derived from dietary sources such as flaxseed, sesame seeds, kale, broccoli, and apricots, may impede tumor proliferation by inhibiting activation of nuclear factor kappa B (NFκB) and vascular endothelial growth factor (VEGF). We examined the associations between urinary enterolactone and enterodiol with prostatic tumor expression of NFκB, VEGF, and Ki67 among 147 patients with prostate cancer who participated in a presurgical trial of flaxseed supplementation (30 g/day) for ∼30 days. Urinary enterolignans and tissue biomarkers were determined by high-performance liquid chromatography and immunohistochemistry, respectively. After supplementation, we observed significant correlations between intakes of plant lignan and urinary concentrations of total enterolignans (ρ=0.677, P<.0001), enterolactone (ρ=0.676, P<.0001), and enterodiol (ρ=0.628, P<.0001). Importantly, we observed that total urinary enterolignans and enterolactone were significantly and inversely correlated with Ki67 in the tumor tissue (ρ=-0.217, P=.011, and ρ=-0.230, P=.007, respectively), and a near-significant inverse association was observed for enterodiol (ρ=-0.159, P=.064). An inverse association was observed between enterolactone and VEGF (ρ=-0.143, P=.141), although this did not reach statistical significance. We did not observe an association between enterolignans and NFκB. In conclusion, flaxseed-derived enterolignans may hinder cancer cell proliferation via VEGF-associated pathways.",
author = "Maria Azrad and Vollmer, {Robin T.} and John Madden and Mark Dewhirst and Polascik, {Thomas J.} and Snyder, {Denise C.} and Ruffin, {Mack T.} and Moul, {Judd W.} and Brenner, {Dean E.} and Wendy Demark-Wahnefried",
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Azrad, M, Vollmer, RT, Madden, J, Dewhirst, M, Polascik, TJ, Snyder, DC, Ruffin, MT, Moul, JW, Brenner, DE & Demark-Wahnefried, W 2013, 'Flaxseed-derived enterolactone is inversely associated with tumor cell proliferation in men with localized prostate cancer', Journal of Medicinal Food, vol. 16, no. 4, pp. 357-360. https://doi.org/10.1089/jmf.2012.0159

Flaxseed-derived enterolactone is inversely associated with tumor cell proliferation in men with localized prostate cancer. / Azrad, Maria; Vollmer, Robin T.; Madden, John; Dewhirst, Mark; Polascik, Thomas J.; Snyder, Denise C.; Ruffin, Mack T.; Moul, Judd W.; Brenner, Dean E.; Demark-Wahnefried, Wendy.

In: Journal of Medicinal Food, Vol. 16, No. 4, 01.04.2013, p. 357-360.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Flaxseed-derived enterolactone is inversely associated with tumor cell proliferation in men with localized prostate cancer

AU - Azrad, Maria

AU - Vollmer, Robin T.

AU - Madden, John

AU - Dewhirst, Mark

AU - Polascik, Thomas J.

AU - Snyder, Denise C.

AU - Ruffin, Mack T.

AU - Moul, Judd W.

AU - Brenner, Dean E.

AU - Demark-Wahnefried, Wendy

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Enterolactone and enterodiol, mammalian lignans derived from dietary sources such as flaxseed, sesame seeds, kale, broccoli, and apricots, may impede tumor proliferation by inhibiting activation of nuclear factor kappa B (NFκB) and vascular endothelial growth factor (VEGF). We examined the associations between urinary enterolactone and enterodiol with prostatic tumor expression of NFκB, VEGF, and Ki67 among 147 patients with prostate cancer who participated in a presurgical trial of flaxseed supplementation (30 g/day) for ∼30 days. Urinary enterolignans and tissue biomarkers were determined by high-performance liquid chromatography and immunohistochemistry, respectively. After supplementation, we observed significant correlations between intakes of plant lignan and urinary concentrations of total enterolignans (ρ=0.677, P<.0001), enterolactone (ρ=0.676, P<.0001), and enterodiol (ρ=0.628, P<.0001). Importantly, we observed that total urinary enterolignans and enterolactone were significantly and inversely correlated with Ki67 in the tumor tissue (ρ=-0.217, P=.011, and ρ=-0.230, P=.007, respectively), and a near-significant inverse association was observed for enterodiol (ρ=-0.159, P=.064). An inverse association was observed between enterolactone and VEGF (ρ=-0.143, P=.141), although this did not reach statistical significance. We did not observe an association between enterolignans and NFκB. In conclusion, flaxseed-derived enterolignans may hinder cancer cell proliferation via VEGF-associated pathways.

AB - Enterolactone and enterodiol, mammalian lignans derived from dietary sources such as flaxseed, sesame seeds, kale, broccoli, and apricots, may impede tumor proliferation by inhibiting activation of nuclear factor kappa B (NFκB) and vascular endothelial growth factor (VEGF). We examined the associations between urinary enterolactone and enterodiol with prostatic tumor expression of NFκB, VEGF, and Ki67 among 147 patients with prostate cancer who participated in a presurgical trial of flaxseed supplementation (30 g/day) for ∼30 days. Urinary enterolignans and tissue biomarkers were determined by high-performance liquid chromatography and immunohistochemistry, respectively. After supplementation, we observed significant correlations between intakes of plant lignan and urinary concentrations of total enterolignans (ρ=0.677, P<.0001), enterolactone (ρ=0.676, P<.0001), and enterodiol (ρ=0.628, P<.0001). Importantly, we observed that total urinary enterolignans and enterolactone were significantly and inversely correlated with Ki67 in the tumor tissue (ρ=-0.217, P=.011, and ρ=-0.230, P=.007, respectively), and a near-significant inverse association was observed for enterodiol (ρ=-0.159, P=.064). An inverse association was observed between enterolactone and VEGF (ρ=-0.143, P=.141), although this did not reach statistical significance. We did not observe an association between enterolignans and NFκB. In conclusion, flaxseed-derived enterolignans may hinder cancer cell proliferation via VEGF-associated pathways.

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