Flow cytofluorometric analysis of insulin binding and internalization by swiss 3T3 cells

Robert F. Murphy, Scott Powers, Michael Verderame, Charles R. Cantor, Robert Pollack

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The binding of a fluorescein‐isothiocyanate derivative of insulin to Swiss 3T3 cells was measured by flow cytometry. The kinetics of the subsequent internalization were also measured; at a concentration of 1 μM labeled insulin approximately 25% of the internalization was insulin‐specific. The kinetics of endocytosis were contrasted to those of fluorescent derivatives of histone and dextran. In addition, the fusion of endocytic vesicles containing insulin or dextran with lysosomes was detected by measuring the pH‐dependent increase in fluorescein fluorescence caused by the addition of chloroquine. The application of these results to the analysis of growth control by insulin and related hormones is discussed.

Original languageEnglish (US)
Pages (from-to)402-406
Number of pages5
JournalCytometry
Volume2
Issue number6
DOIs
StatePublished - May 1982

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

Fingerprint Dive into the research topics of 'Flow cytofluorometric analysis of insulin binding and internalization by swiss 3T3 cells'. Together they form a unique fingerprint.

  • Cite this