Flow cytometry demonstrates bacteriocin-induced injury to Listeria monocytogenes

Avril J. Swarts, John W. Hastings, Robert F. Roberts, Alexander Von Holy

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Flow cytometry was used to study the effect of the bacteriocin leucocin B-TA11a on Listeria (L.) monocytogenes. Mixed proportions of dead and live control populations were analyzed by flow cytometry to determine detection limits of the Dead/Live Baclight Bacterial Viability Kit(TM). High correlations for flow cytometric detection of defined proportions of live or dead cells in mixtures between 10 and 100% of dead (r2 = 0.97) or live (r2 = 0.99) cells were obtained. However, mixtures containing less than 10% of either live or dead control cells gave correlations below 0.72. The growth of L. monocytogenes in the absence and presence of leucocin B-TA11a was analyzed by flow cytometry with Baclight, plate counts, and optical density measurements. Although leucocin B-TA11a initially inhibited listerial growth, the uptake of both Baclight dyes suggested that cells remained viable but became leaky, possibly indicating bacteriocin-induced pore formation in the target membranes.

Original languageEnglish (US)
Pages (from-to)266-270
Number of pages5
JournalCurrent Microbiology
Volume36
Issue number5
DOIs
StatePublished - 1998

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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