Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus

Andrea E. Granstedt, Moriah L. Szpara, Bernd Kuhn, Samuel S.H. Wang, Lynn W. Enquist

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The study of coordinated activity in neuronal circuits has been challenging without a method to simultaneously report activity and connectivity. Here we present the first use of pseudorabies virus (PRV), which spreads through synaptically connected neurons, to express a fluorescent calcium indicator protein and monitor neuronal activity in a living animal. Fluorescence signals were proportional to action potential number and could reliably detect single action potentials in vitro. With two-photon imaging in vivo, we observed both spontaneous and stimulated activity in neurons of infected murine peripheral autonomic submandibular ganglia (SMG). We optically recorded the SMG response in the salivary circuit to direct electrical stimulation of the presynaptic axons and to physiologically relevant sensory stimulation of the oral cavity. During a time window of 48 hours after inoculation, few spontaneous transients occurred. By 72 hours, we identified more frequent and prolonged spontaneous calcium transients, suggestive of neuronal or tissue responses to infection that influence calcium signaling. Our work establishes in vivo investigation of physiological neuronal circuit activity and subsequent effects of infection with single cell resolution.

Original languageEnglish (US)
Article numbere6923
JournalPloS one
Volume4
Issue number9
DOIs
StatePublished - Sep 9 2009

Fingerprint

Parasympathetic Ganglia
Suid Herpesvirus 1
Suid herpesvirus 1
action potentials
Viruses
Action Potentials
Fluorescence
neurons
Autonomic Ganglia
fluorescence
Calcium
Neurons
calcium
Calcium Signaling
Networks (circuits)
Monitoring
monitoring
Infection
Photons
axons

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Granstedt, Andrea E. ; Szpara, Moriah L. ; Kuhn, Bernd ; Wang, Samuel S.H. ; Enquist, Lynn W. / Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus. In: PloS one. 2009 ; Vol. 4, No. 9.
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Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus. / Granstedt, Andrea E.; Szpara, Moriah L.; Kuhn, Bernd; Wang, Samuel S.H.; Enquist, Lynn W.

In: PloS one, Vol. 4, No. 9, e6923, 09.09.2009.

Research output: Contribution to journalArticle

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