Fluorescent amplified fragment length polymorphism subtyping of multiresistant Salmonella enterica serovar Typhimurium DT104

Andrew J. Lawson, John Stanley, E. John Threlfall, Meeta Desai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fluorescent amplified fragment length polymorphism (FAFLP) subtyping analysis was used to genotype multiresistant Salmonella enterica serovar Typhimurium definitive phage type 104. Thirteen distinct FAFLP profiles were found among 85 isolates exhibiting identical pulsed-fleld gel electrophoresis (PFGE) profiles. A single FAFLP profile was shared by 93% of outbreak-associated isolates and 82% of sporadic isolates. This study demonstrates the value of FAFLP as a high-resolution tool for epidemiological investigation of Salmonella.

Original languageEnglish (US)
Pages (from-to)4843-4845
Number of pages3
JournalJournal of clinical microbiology
Volume42
Issue number10
DOIs
StatePublished - Oct 1 2004

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Amplified Fragment Length Polymorphism Analysis
Salmonella enterica
Salmonella
Bacteriophages
Disease Outbreaks
Electrophoresis
Gels
Genotype
Serogroup

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

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Fluorescent amplified fragment length polymorphism subtyping of multiresistant Salmonella enterica serovar Typhimurium DT104. / Lawson, Andrew J.; Stanley, John; Threlfall, E. John; Desai, Meeta.

In: Journal of clinical microbiology, Vol. 42, No. 10, 01.10.2004, p. 4843-4845.

Research output: Contribution to journalArticle

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T1 - Fluorescent amplified fragment length polymorphism subtyping of multiresistant Salmonella enterica serovar Typhimurium DT104

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